MAPRE1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84928

Novus Biologicals, part of Bio-Techne
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NBP1-84928
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MAPRE1 Antibodies »

Product Summary for MAPRE1 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VAPSLVAPALNKPKKPLTSSSAAPQRPISTQRTAAAPKAGPGVVRKNPGVGNGDDEAAELMQQVNVLKLTVEDLEKERDFYFGKLRNIELICQENEGENDPVLQRIVDILYATDEGFVIPDE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MAPRE1 Antibody

Western Blot: MAPRE1 Antibody [NBP1-84928]

Western Blot: MAPRE1 Antibody [NBP1-84928]

Western Blot: MAPRE1 Antibody [NBP1-84928] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: MAPRE1 Antibody [NBP1-84928]

Immunocytochemistry/ Immunofluorescence: MAPRE1 Antibody [NBP1-84928]

Immunocytochemistry/Immunofluorescence: MAPRE1 Antibody [NBP1-84928] - Staining of human cell line U-251 MG shows localization to cytosol and microtubule organizing center. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: MAPRE1 Antibody [NBP1-84928]

Immunohistochemistry-Paraffin: MAPRE1 Antibody [NBP1-84928]

Immunohistochemistry-Paraffin: MAPRE1 Antibody [NBP1-84928] - Staining in human testis and skeletal muscle tissues using anti-MAPRE1 antibody. Corresponding MAPRE1 RNA-seq data are presented for the same tissues.
View 3 more images

Applications for MAPRE1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MAPRE1

End -binding protein 1 (EB1) is a member of the RP/EB family and was first identified by its binding to the APC protein which is often mutated in familial and sporadic forms of colorectal cancer. This protein localizes to microtubules, especially the growing ends, in interphase cells. During mitosis, the protein is associated with the centrosomes and spindle microtubules. EB1 also associates with components of the dynactin complex and the intermediate chain of cytoplasmic dynein. Because of these associations, it is thought that EB1 is involved in the regulation of microtubule structures and chromosome stability. [rom NCBI GeneID:22919]

Alternate Names

adenomatous polyposis coli-binding protein EB1, APC-binding protein EB1, EB1MGC129946, End-binding protein 1, MGC117374, microtubule-associated protein RP/EB family member 1, microtubule-associated protein, RP/EB family, member 1

Gene Symbol

MAPRE1

Additional MAPRE1 Products

Product Documents for MAPRE1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MAPRE1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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