MARCKS Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-68861

Novus Biologicals, part of Bio-Techne
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NBP2-68861
NBP2-68861-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MARCKS Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: DPQEAKPQEAAVAPEKPPASDETKAAEEPSKVEEK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MARCKS Antibody

Immunocytochemistry/ Immunofluorescence: MARCKS Antibody [NBP2-68861]

Immunocytochemistry/ Immunofluorescence: MARCKS Antibody [NBP2-68861]

Immunocytochemistry/Immunofluorescence: MARCKS Antibody [NBP2-68861] - Immunohistochemical staining of human colon shows strong cytoplasmic and membranous positivity in glandular cells.
Immunocytochemistry/ Immunofluorescence: MARCKS Antibody [NBP2-68861]

Immunocytochemistry/ Immunofluorescence: MARCKS Antibody [NBP2-68861]

Immunocytochemistry/Immunofluorescence: MARCKS Antibody [NBP2-68861] - Staining of human cell line U-251 MG shows localization to plasma membrane & endoplasmic reticulum.

Applications for MARCKS Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000
Application Notes
Recommended conditions for ICC/IF: Fixation/Permeabilization: PFA/Triton X-100, Recommended conditions for IHC: Retrieval method: HIER pH6

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MARCKS

Myristolyated alanine rich C-kinase substrate, hence MARCKS, was originally discovered as a major substrate for protein kinase C in the brain and other tissues, and was originally isolated from human epithelial cells. MARCKS is a major protein of brain, and is concentrated in synapses of neurons. It appears to function in synaptic vesicle cycling and has been shown to bind both actin and calmodulin in vitro. MARCKS belongs to a family of proteins with similar actin and calmodulin binding properties. Deletion of the MARCKS gene in mice results in embryonic brain defects and death.

Long Name

Myristoylated Alanine-rich Protein Kinase C Substrate

Alternate Names

80K-L, MACS, PRKCSL

Gene Symbol

MARCKS

Additional MARCKS Products

Product Documents for MARCKS Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MARCKS Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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