MARCKS [p Ser158] Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05422

Novus Biologicals, part of Bio-Techne
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NBP3-05422
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MARCKS Antibodies »

Product Specifications

Immunogen

A phospho specific peptide corresponding to residues surrounding S158 of human MARCKS

Modification

p Ser158

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MARCKS [p Ser158] Antibody - BSA Free

Western Blot: MARCKS [p Ser158] Antibody [NBP3-05422] - Western blot analysis of extracts from 3T3 cells using MARCKS antibody (NBP3-05422). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.
Immunocytochemistry/ Immunofluorescence: MARCKS [p Ser158] Antibody - BSA Free [NBP3-05422]

Immunocytochemistry/ Immunofluorescence: MARCKS [p Ser158] Antibody - BSA Free [NBP3-05422]

Immunocytochemistry/Immunofluorescence: MARCKS [p Ser158] Antibody [NBP3-05422] - Immunofluorescence analysis of methanol-fixed HeLa cells using MARCKS antibody (NBP3-05422).

Applications for MARCKS [p Ser158] Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MARCKS

Myristolyated alanine rich C-kinase substrate, hence MARCKS, was originally discovered as a major substrate for protein kinase C in the brain and other tissues, and was originally isolated from human epithelial cells. MARCKS is a major protein of brain, and is concentrated in synapses of neurons. It appears to function in synaptic vesicle cycling and has been shown to bind both actin and calmodulin in vitro. MARCKS belongs to a family of proteins with similar actin and calmodulin binding properties. Deletion of the MARCKS gene in mice results in embryonic brain defects and death.

Long Name

Myristoylated Alanine-rich Protein Kinase C Substrate

Alternate Names

80K-L, MACS, PRKCSL

Gene Symbol

MARCKS

Additional MARCKS Products

Product Documents for MARCKS [p Ser158] Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MARCKS [p Ser158] Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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