MARK4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83442

Novus Biologicals, part of Bio-Techne
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NBP1-83442
NBP1-83442-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (90%), Rat (92%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MARK4 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PSDTTNGTSSSKGTSHSKGQRSSSSTYHRQRRHSDFCGPSPAPLHPKRSPTSTGEAELKEERLPGRKASCSTAGSGSR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MARK4 Antibody

Immunocytochemistry/ Immunofluorescence: MARK4 Antibody [NBP1-83442]

Immunocytochemistry/ Immunofluorescence: MARK4 Antibody [NBP1-83442]

Immunocytochemistry/Immunofluorescence: MARK4 Antibody [NBP1-83442] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
Immunohistochemistry-Paraffin: MARK4 Antibody [NBP1-83442]

Immunohistochemistry-Paraffin: MARK4 Antibody [NBP1-83442]

Immunohistochemistry-Paraffin: MARK4 Antibody [NBP1-83442] - Staining of human colon shows moderate cytoplasmic positivity in glandular cells.

Applications for MARK4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MARK4

MARK4 contains an N terminal serine/threonine kinase domain, a central ubiquitin associated domain, and a C terminal KA1 associated kinase domain. RT PCR analysis detects upregulated expression of the gene for MARK4 in nearly all clinical hepatocellular carcinoma cells. Northern blot analysis reveals ubiquitous expression of a 3.6kb transcript, with highest expression in testis. Immunofluorescence microscopy demonstrates homogeneous cytoplasmic expression. Colony forming assays show that MARK4 antisense reduces the growth of SNU475 cells. It has been suggested that MARK4 provides a growth advantage to cells.

Alternate Names

EC 2.7.11, EC 2.7.11.1, FLJ12177, FLJ90097, KIAA1860MARK4L, MAP/microtubule affinity-regulating kinase 4, MAP/microtubule affinity-regulating kinase like 1, MAP/microtubule affinity-regulating kinase-like 1, MARK4 serine/threonine protein kinase, MARKL1L, MARKL1MARK4S, Nbla00650

Gene Symbol

MARK4

Additional MARK4 Products

Product Documents for MARK4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MARK4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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