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MAT1/2A Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB110-94162

Novus Biologicals, part of Bio-Techne
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NB110-94162
NB110-94162SS

Key Product Details

Species Reactivity

Validated:

Human, Rat, Bovine, Mouse (Negative), Primate, Zebrafish

Cited:

Mouse

Applications

Validated:

Simple Western, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Synthetic peptide made to an internal portion of the human MAT2A protein (within residues 100-200). [Swiss-Prot# P31153]

Reactivity Notes

Orangutan. Does not react with mouse.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MAT1/2A Antibody - BSA Free

Western Blot: MAT1/2A AntibodyBSA Free [NB110-94162]

Western Blot: MAT1/2A AntibodyBSA Free [NB110-94162]

Western Blot: MAT1/2A Antibody [NB110-94162] - Detection of MAT2A in HepG2 whole cell lysates using NB110-94162.
Simple Western: MAT1/2A AntibodyBSA Free [NB110-94162]

Simple Western: MAT1/2A AntibodyBSA Free [NB110-94162]

Simple Western: MAT1/2A Antibody [NB110-94162] - Simple Western lane view shows a specific band for MAT1/2 A in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Applications for MAT1/2A Antibody - BSA Free

Application
Recommended Usage

Simple Western

1:10

Western Blot

2 ug/ml
Application Notes
This MAT1/2A antibody is useful for Western blot, where a band is seen at approx. 43 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reviewed Applications

Read 1 review rated 5 using NB110-94162 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MAT1/2A

MAT1A and MAT2A are catalytic subunits of methionine adenosyltransferase (MAT), which catalyzes the formation of S-adenosyltransferase for methionine catabolism. Inactivation of MAT1A has been linked to liver disease. In addition, in certain liver cancers expression of MAT1A is replaced by expression of MAT1B. Overexpression of MAT2A has also been observed in colon cancer, leading to the proposal that MAT2A facilitates cancer cell growth.

Alternate Names

AdoMet synthase 2, AMS2, EC 2.5.1.6, MAT 2, MATA2SAMS2adoMet synthetase 2, MATII, MAT-II, Methionine adenosyltransferase 2, Methionine adenosyltransferase II, methionine adenosyltransferase II, alpha, S-adenosylmethionine synthase isoform type-2, S-adenosylmethionine synthetase isoform type-2

Gene Symbol

MAT2A

Additional MAT1/2A Products

Product Documents for MAT1/2A Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MAT1/2A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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