MAT1A Antibody (4D11)

Novus Biologicals, part of Bio-Techne | Catalog # H00004143-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, C. elegans

Cited:

Nematode - Caenorhabditis elegans

Applications

Validated:

ELISA, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4D11

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MAT1A Antibodies »

Product Specifications

Immunogen

MAT1A (AAH18359, 1 a.a. ~ 395 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MNGPVDGLCDHSLSEGVFMFTSESVGEGHPDKICDQISDAVLDAHLKQDPNAKVACETVCKTGMVLLCGEITSMAMVDYQRVVRDTIKHIGYDDSAKGFDFKTCNVLVALEQQSPDIAQCVHLDRNEEDVGAGDQGLMFGYATDETEECMPLTIILAHKLNARMADLRRSGLLPWLRPDSKTQVTVQYMQDNGAVIPVRIHTIVISVQHNEDITLEEMRRALKEQVIRAVVPAKYLDEDTVYHLQPSGRFVIGGPQGDAGVTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAEPLSISIFTYGTSQKTERELLDVVHKNFDLRPGVIVRDLDLKKPIYQKTACYGHFGRSEFPWEVPRKLVF

Reactivity Notes

C. elegans reactivity reported in scientific literature (PMID: 26321661).

Specificity

MAT1A - methionine adenosyltransferase I, alpha (4D11)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for MAT1A Antibody (4D11)

Western Blot: MAT1A Antibody (4D11) [H00004143-M01]

Western Blot: MAT1A Antibody (4D11) [H00004143-M01]

Western Blot: MAT1A Antibody (4D11) [H00004143-M01] - Analysis of MAT1A expression in transfected 293T cell line by MAT1A monoclonal antibody (M01), clone 4D11.Lane 1: MAT1A transfected lysate(43.6 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation: MAT1A Antibody (4D11) [H00004143-M01]

Immunoprecipitation: MAT1A Antibody (4D11) [H00004143-M01]

Immunoprecipitation: MAT1A Antibody (4D11) [H00004143-M01] - Analysis of MAT1A transfected lysate using anti-MAT1A monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MAT1A monoclonal antibody.
ELISA: MAT1A Antibody (4D11) [H00004143-M01]

ELISA: MAT1A Antibody (4D11) [H00004143-M01]

ELISA: MAT1A Antibody (4D11) [H00004143-M01] - Detection limit for recombinant GST tagged MAT1A is 1 ng/ml as a capture antibody.

Applications for MAT1A Antibody (4D11)

Application
Recommended Usage

Immunoprecipitation

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MAT1A

This gene catalyzes a two-step reaction that involves the transfer of the adenosyl moiety of ATP to methionine to form S-adenosylmethionine and tripolyphosphate, which is subsequently cleaved to PPi and Pi. S-adenosylmethionine is the source of methyl groups for most biological methylations. The encoded protein is found as a homotetramer (MAT I) or a homodimer (MAT III) whereas a third form, MAT II (gamma), is encoded by the MAT2A gene. Mutations in this gene are associated with methionine adenosyltransferase deficiency. [provided by RefSeq]

Alternate Names

AdoMet synthase 1, adoMet synthetase 1, AMS1, MAT, MATA1MAT 1, MAT-I/III, Methionine adenosyltransferase 1, methionine adenosyltransferase I, alpha, Methionine adenosyltransferase I/III, S-adenosylmethionine synthase isoform type-1, S-adenosylmethionine synthetase isoform type-1, SAMS, SAMS1EC 2.5.1.6

Entrez Gene IDs

4143 (Human)

Gene Symbol

MAT1A

UniProt

AAH18359 (Human)

Additional MAT1A Products

Product Documents for MAT1A Antibody (4D11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MAT1A Antibody (4D11)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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