MAT2A Antibody (3A2) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-28605

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 3A2

Format

BSA Free

Concentration

1.0 mg/ml

View all MAT2A Antibodies »

Product Specifications

Immunogen

Recombinant human MAT2A (1-395aa) purified from E. coli (NP_005902).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MAT2A Antibody (3A2) - BSA Free

Western Blot: MAT2A Antibody (3A2)BSA Free [NBP1-28605]

Western Blot: MAT2A Antibody (3A2)BSA Free [NBP1-28605]

Western Blot: MAT2A Antibody (3A2) [NBP1-28605] - The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MAT2A antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1. : HeLa cell lysateLane 2. : Ramos cell lysateLane 3. : 293T cell lysateLane 4. : Jurkat cell lysateLane 5. : NIH-3T3 cell lysate
Immunocytochemistry/ Immunofluorescence: MAT2A Antibody (3A2) - BSA Free [NBP1-28605]

Immunocytochemistry/ Immunofluorescence: MAT2A Antibody (3A2) - BSA Free [NBP1-28605]

Immunocytochemistry/Immunofluorescence: MAT2A Antibody (3A2) [NBP1-28605] - Analysis of MAT2A in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human MAT2A antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Immunohistochemistry-Paraffin: MAT2A Antibody (3A2) - BSA Free [NBP1-28605]

Immunohistochemistry-Paraffin: MAT2A Antibody (3A2) - BSA Free [NBP1-28605]

Immunohistochemistry-Paraffin: MAT2A Antibody (3A2) [NBP1-28605] - Human MAT2A (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).
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Applications for MAT2A Antibody (3A2) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:50

Western Blot

1:1000-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MAT2A

S adenosylmethionine synthetase catalyzes the formation of S adenosylmethionine from methionine and ATP. In mammalian tissues, there are three distinct forms of AdoMet synthases designated as alpha, beta, and gamma. Alpha and beta are expressed only in adult liver, while gamma is widely distributed in extrahepatic tissues.

Long Name

Methionine Adenosyltransferase 2A

Alternate Names

MAT-II, MATA2, SAMS2

Entrez Gene IDs

4144 (Human)

Gene Symbol

MAT2A

UniProt

P31153 (Human)

Additional MAT2A Products

Product Documents for MAT2A Antibody (3A2) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MAT2A Antibody (3A2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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