MAVS Antibody (PSH01-65)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32570

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # PSH01-65

Concentration

1 mg/ml

View all MAVS Antibodies »

Product Summary for MAVS Antibody (PSH01-65)

Immunogen

Synthetic peptide within human MAVS aa 450-504 (Cytoplasmic). (Uniprot: Q7Z434)

Localization

Membrane, Mitochondrion, Mitochondrion outer membrane, Peroxisome

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

56.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MAVS Antibody (PSH01-65)

MAVS Antibody (PSH01-65)

Western Blot: MAVS Antibody (PSH01-65) [NBP3-32570]

Western Blot: MAVS Antibody (PSH01-65) [NBP3-32570] - Western blot analysis of MAVS on different lysates with Rabbit anti-MAVS antibody (NBP3-32570) at 1/1,000 dilution.

Lane 1: MCF7 cell lysate
Lane 2: A431 cell lysate
Lane 3: A549 cell lysate
Lane 4: Jurkat cell lysate
Lane 5: THP-1 cell lysate
Lane 6: HepG2 cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 56.5 kDa
Observed band size: 57/75 kDa

Exposure time: 1 minute 46 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32570) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.
MAVS Antibody (PSH01-65)

Immunohistochemistry: MAVS Antibody (PSH01-65) [NBP3-32570]

Immunohistochemistry: MAVS Antibody (PSH01-65) [NBP3-32570] - Immunofluorescence analysis of paraffin-embedded human colon tissue labeling MAVS with Rabbit anti-MAVS antibody (NBP3-32570) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32570, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
MAVS Antibody (PSH01-65)

Immunohistochemistry: MAVS Antibody (PSH01-65) [NBP3-32570]

Immunohistochemistry: MAVS Antibody (PSH01-65) [NBP3-32570] - Immunohistochemical analysis of paraffin-embedded human kideny tissue with Rabbit anti-MAVS antibody (NBP3-32570) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32570) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Applications for MAVS Antibody (PSH01-65)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MAVS

Double-stranded RNA viruses are recognized in a cell type-dependent manner by the transmembrane receptor TLR3 (MIM 603029) or by the cytoplasmic RNA helicases MDA5 (MIM 606951) and RIGI (ROBO3; MIM 608630). These interactions initiate signaling pathways that differ in their initial steps but converge in the activation of the protein kinases IKKA (CHUK; MIM 600664) and IKKB (IKBKB; MIM 603258), which activate NFKB (see MIM 164011), or TBK1 (MIM 604834) and IKKE (IKBKE; MIM 605048), which activate IRF3 (MIM 603734). Activated IRF3 and NFKB induce transcription of IFNB (IFNB1; MIM 147640). For the TLR3 pathway, the intermediary molecule before the pathways converge is the cytoplasmic protein TRIF (TICAM1; MIM 607601). For RIGI, the intermediary protein is mitochondria-bound IPS1 (Sen and Sarkar, 2005).[supplied by OMIM]

Long Name

Mitochondrial Antiviral Signaling Protein

Alternate Names

Cardif, IPS-1, VISA

Gene Symbol

MAVS

Additional MAVS Products

Product Documents for MAVS Antibody (PSH01-65)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MAVS Antibody (PSH01-65)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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