MBD1 Antibody (100B272.1) [Janelia Fluor® 635]

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56537JF635

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Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Unconjugated

NB100-56537

Alexa Fluor 350

NB100-56537AF350

Alexa Fluor 405

NB100-56537AF405

Alexa Fluor 488

NB100-56537AF488

Alexa Fluor 532

NB100-56537AF532

Alexa Fluor 594

NB100-56537AF594

Alexa Fluor 647

NB100-56537AF647

Alexa Fluor 700

NB100-56537AF700

Alexa Fluor 750

NB100-56537AF750

Biotin

NB100-56537B

CoraFluor 1

NB100-56537CL1

DyLight 350

NB100-56537UV

DyLight 405

NB100-56537V

DyLight 488

NB100-56537G

DyLight 550

NB100-56537R

DyLight 594

NB100-56537DL594

DyLight 650

NB100-56537C

DyLight 680

NB100-56537FR

DyLight 755

NB100-56537IR

FITC

NB100-56537F

HRP

NB100-56537H

Janelia Fluor 525

NB100-56537JF525

Janelia Fluor 549

NB100-56537JF549

Janelia Fluor 585

NB100-56537JF585

Janelia Fluor 646

NB100-56537JF646

Janelia Fluor 669

NB100-56537JF669

mFluor Violet 450 SE

NB100-56537MFV450

mFluor Violet 500 SE

NB100-56537MFV500

mFluor Violet 610 SE

NB100-56537MFV610

Forumulation

Catalog #

Azide and BSA Free

NBP2-33221

Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse

Applications

Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockdown Validated, Western Blot

Label

Janelia Fluor 635

Antibody Source

Monoclonal Mouse IgG₁ Clone # 100B272.1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MBD1 Antibodies »

Product Specifications

Immunogen

This antibody was generated by immunizing mice with a synthetic peptide corresponding to amino acids 391-405 (SESEDGAGSPPPYRR) of human MBD1; GenBank no ref|NP_056671.2|

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25798578)

Specificity

There are many isoforms of human MBD1 ranging from 503 (~55 kDa) to 655 (~71 kDa) amino acids in length. Researchers are encouraged to use the provided immunogen sequence to determine if this antibody is suitable for testing a specific isoform of interest.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Applications for MBD1 Antibody (100B272.1) [Janelia Fluor® 635]

Application

Recommended Usage

Chromatin Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Chromatin Immunoprecipitation (ChIP)

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: MBD1

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. Five transcript variants of the MBD1 are generated by alternative splicing resulting in protein isoforms that contain one MBD domain, two to three cysteine-rich (CXXC) domains, and some differences in the COOH terminus. All five transcript variants repress transcription from methylated promoters; in addition, variants with three CXXC domains also repress unmethylated promoter activity. MBD1 and MBD2 map very close to each other on chromosome 18q21.

Alternate Names

CXXC-type zinc finger protein 3, methyl-CpG binding domain protein 1, methyl-CpG binding domain protein 1 isoform PCM1, methyl-CpG-binding domain protein 1, Methyl-CpG-binding protein MBD1, PCM1CXXC3RFT, Protein containing methyl-CpG-binding domain 1, the regulator of fibroblast growth factor 2 (FGF-2) transcription

Gene Symbol

MBD1

Additional MBD1 Products

Product Documents for MBD1 Antibody (100B272.1) [Janelia Fluor® 635]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for MBD1 Antibody (100B272.1) [Janelia Fluor® 635]

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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