Key Product Details

Species Reactivity

Mouse

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry

Label

Biotin

Antibody Source

Recombinant Monoclonal Rat IgG2A Clone # 733216

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MCAM/CD146 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse MCAM/CD146
Met1-Val563
Accession # Q8R2Y2

Specificity

Detects mouse MCAM/CD146 in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse (rm) MAdCAM-1 is observed, and no cross-reactivity with rmALCAM, rmNCAM, rmL1CAM, rmOCAM, rmTROP-2, recombinant human MCAM, or recombinant rat MCAM is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for MCAM/CD146 Antibody (733216) [Biotin]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: MCAM/CD146

CD146 belongs to the immunoglobulin supergene family with five immunoglobulin like domains (V-V-C2-C2-C2), a transmembrane region and a 63 residue cytoplasmic tail. The protein is a membrane glycoprotein that functions as a Ca2+ independent cell adhesion molecule involved in heterophilic cell to cell interactions. CD146 has a molecular size of 130 kDa in its reduced form (118 kDa unreduced), and N linked glycosylation accounts for fifty percent of the apparent molecular weight. In some cells the molecule carries a sulfate 3 glucuronyl moiety. Expression of the molecule was shown in a relatively limited spectrum of normal human tissues (endothelium, smooth muscle, and subpopulations of activated T cells) and malignant neoplasm (melanoma and breast carcinoma). The lineage specific expression pattern of CD146 can be useful in the differential diagnosis of certain lesions including melanomas and various types of gestational trophoblastic lesions. CD146 expression can promote tumor progression in human melanoma, through enhanced interaction between melanoma cells and endothelial cells. However, in breast carcinoma, CD146 may act as a tumor suppressor. Over expression of CD146 in breast carcinoma cells results in a more cohesive cell growth and in the formation of smaller tumors in nude mice. During implantation and placentation, CD146 is expressed by the intermediate trophoblast in the placental site and binds to its putative receptor in uterine smooth muscle cells thus limiting trophoblastic invasion in the myometrium. Monoclonal antibody specific for CD146 is an important tool for the identification and isolation of cells expressing CD146.

Long Name

Melanoma Cell Adhesion Molecule

Alternate Names

CD146, L-Gicerin, MUC18

Gene Symbol

MCAM

Additional MCAM/CD146 Products

Product Documents for MCAM/CD146 Antibody (733216) [Biotin]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MCAM/CD146 Antibody (733216) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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