MCAM/CD146 Antibody (MCAM/1101) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47776

Novus Biologicals, part of Bio-Techne
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NBP2-47776-0.1mg
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # MCAM/1101

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all MCAM/CD146 Antibodies »

Product Specifications

Immunogen

Recombinant human MCAM/CD146 protein (Uniprot: P43121)

Localization

Cell surface

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

130 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44509).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for MCAM/CD146 Antibody (MCAM/1101) - Azide and BSA Free

Immunohistochemistry-Paraffin: MCAM/CD146 Antibody (MCAM/1101) - Azide and BSA Free [NBP2-47776]

Immunohistochemistry-Paraffin: MCAM/CD146 Antibody (MCAM/1101) - Azide and BSA Free [NBP2-47776]

Immunohistochemistry-Paraffin: MCAM/CD146 Antibody (MCAM/1101) - Azide and BSA Free [NBP2-47776] - Human Melanoma stained with MCAM Monoclonal Antibody (MCAM/1101)
Immunohistochemistry-Paraffin: MCAM/CD146 Antibody (MCAM/1101) - Azide and BSA Free [NBP2-47776]

Immunohistochemistry-Paraffin: MCAM/CD146 Antibody (MCAM/1101) - Azide and BSA Free [NBP2-47776]

Immunohistochemistry-Paraffin: MCAM/CD146 Antibody (MCAM/1101) - Azide and BSA Free [NBP2-47776] - Human Melanoma stained with MCAM Monoclonal Antibody (MCAM/1101)
Immunohistochemistry-Paraffin: MCAM/CD146 Antibody (MCAM/1101) - Azide and BSA Free [NBP2-47776]

Immunohistochemistry-Paraffin: MCAM/CD146 Antibody (MCAM/1101) - Azide and BSA Free [NBP2-47776]

Immunohistochemistry-Paraffin: MCAM/CD146 Antibody (MCAM/1101) - Azide and BSA Free [NBP2-47776] - Human Tonsil stained with MCAM Monoclonal Antibody (MCAM/1101)

Applications for MCAM/CD146 Antibody (MCAM/1101) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5 - 1 ug/million cells in 0.1 ml

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunofluorescence

0.5 - 1.0 ug/ml

Immunohistochemistry-Paraffin

0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 8.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: MCAM/CD146

CD146 belongs to the immunoglobulin supergene family with five immunoglobulin like domains (V-V-C2-C2-C2), a transmembrane region and a 63 residue cytoplasmic tail. The protein is a membrane glycoprotein that functions as a Ca2+ independent cell adhesion molecule involved in heterophilic cell to cell interactions. CD146 has a molecular size of 130 kDa in its reduced form (118 kDa unreduced), and N linked glycosylation accounts for fifty percent of the apparent molecular weight. In some cells the molecule carries a sulfate 3 glucuronyl moiety. Expression of the molecule was shown in a relatively limited spectrum of normal human tissues (endothelium, smooth muscle, and subpopulations of activated T cells) and malignant neoplasm (melanoma and breast carcinoma). The lineage specific expression pattern of CD146 can be useful in the differential diagnosis of certain lesions including melanomas and various types of gestational trophoblastic lesions. CD146 expression can promote tumor progression in human melanoma, through enhanced interaction between melanoma cells and endothelial cells. However, in breast carcinoma, CD146 may act as a tumor suppressor. Over expression of CD146 in breast carcinoma cells results in a more cohesive cell growth and in the formation of smaller tumors in nude mice. During implantation and placentation, CD146 is expressed by the intermediate trophoblast in the placental site and binds to its putative receptor in uterine smooth muscle cells thus limiting trophoblastic invasion in the myometrium. Monoclonal antibody specific for CD146 is an important tool for the identification and isolation of cells expressing CD146.

Long Name

Melanoma Cell Adhesion Molecule

Alternate Names

CD146, L-Gicerin, MUC18

Gene Symbol

MCAM

Additional MCAM/CD146 Products

Product Documents for MCAM/CD146 Antibody (MCAM/1101) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MCAM/CD146 Antibody (MCAM/1101) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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