MCAM/CD146 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88132

Novus Biologicals, part of Bio-Techne
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NBP1-88132
NBP1-88132-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MCAM/CD146 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LNYRLPSGNHMKESREVTVPVFYPTEKVWLEVEPVGMLKEGDRVEIRCLADGNPPPHFSISKQNPSTREAEEETTNDNGVLVLEPARKEHSGRYECQGLDLDTMISLLSEPQELLVNYVSDVRVSPAAPERQEGSSLTLTCEAESSQDLE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MCAM/CD146 Antibody - BSA Free

Immunohistochemistry-Paraffin: MCAM/CD146 Antibody [NBP1-88132]

Immunohistochemistry-Paraffin: MCAM/CD146 Antibody [NBP1-88132]

Immunohistochemistry-Paraffin: MCAM/CD146 Antibody [NBP1-88132] - Analysis in human placenta and liver tissues using NBP1-88132 antibody. Corresponding MCAM/CD146 RNA-seq data are presented for the same tissues.
Western Blot: MCAM/CD146 Antibody [NBP1-88132]

Western Blot: MCAM/CD146 Antibody [NBP1-88132]

Western Blot: MCAM/CD146 Antibody [NBP1-88132] - Analysis in human cell lines SK-MEL-30 and MCF-7 using anti-MCAM antibody. Corresponding MCAM RNA-seq data are presented for the same cell lines. Loading control: anti-PFN1.
Immunohistochemistry-Paraffin: MCAM/CD146 Antibody [NBP1-88132]

Immunohistochemistry-Paraffin: MCAM/CD146 Antibody [NBP1-88132]

Immunohistochemistry-Paraffin: MCAM/CD146 Antibody [NBP1-88132] - Staining of human kidney shows moderate membranous positivity in cells in glomeruli.
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Applications for MCAM/CD146 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MCAM/CD146

CD146 belongs to the immunoglobulin supergene family with five immunoglobulin like domains (V-V-C2-C2-C2), a transmembrane region and a 63 residue cytoplasmic tail. The protein is a membrane glycoprotein that functions as a Ca2+ independent cell adhesion molecule involved in heterophilic cell to cell interactions. CD146 has a molecular size of 130 kDa in its reduced form (118 kDa unreduced), and N linked glycosylation accounts for fifty percent of the apparent molecular weight. In some cells the molecule carries a sulfate 3 glucuronyl moiety. Expression of the molecule was shown in a relatively limited spectrum of normal human tissues (endothelium, smooth muscle, and subpopulations of activated T cells) and malignant neoplasm (melanoma and breast carcinoma). The lineage specific expression pattern of CD146 can be useful in the differential diagnosis of certain lesions including melanomas and various types of gestational trophoblastic lesions. CD146 expression can promote tumor progression in human melanoma, through enhanced interaction between melanoma cells and endothelial cells. However, in breast carcinoma, CD146 may act as a tumor suppressor. Over expression of CD146 in breast carcinoma cells results in a more cohesive cell growth and in the formation of smaller tumors in nude mice. During implantation and placentation, CD146 is expressed by the intermediate trophoblast in the placental site and binds to its putative receptor in uterine smooth muscle cells thus limiting trophoblastic invasion in the myometrium. Monoclonal antibody specific for CD146 is an important tool for the identification and isolation of cells expressing CD146.

Long Name

Melanoma Cell Adhesion Molecule

Alternate Names

CD146, L-Gicerin, MUC18

Gene Symbol

MCAM

Additional MCAM/CD146 Products

Product Documents for MCAM/CD146 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MCAM/CD146 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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