MCAT Antibody (2F2)

Novus Biologicals, part of Bio-Techne | Catalog # H00027349-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2F2

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MCAT Antibodies »

Product Specifications

Immunogen

MT (NP_775738, 291 a.a. ~ 390 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KKPLVSVYSNVHAHRYRHPGHIHKLLAQQLVSPVKWEQTMHAIYERKKGRGFPQTFEVGPGRQLGAILKSCNMQAWKSYSAVDVLQTLEHVDLDPQEPPR

Reactivity Notes

Human. Other species not tested.

Localization

Mitochondrial

Specificity

MT - malonyl-CoA:acyl carrier protein transacylase, mitochondrial

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for MCAT Antibody (2F2)

Western Blot: MCAT Antibody (2F2) [H00027349-M01]

Western Blot: MCAT Antibody (2F2) [H00027349-M01]

Western Blot: MCAT Antibody (2F2) [H00027349-M01] - MT monoclonal antibody (M01), clone 2F2 Analysis of MT expression in HeLa.
Immunohistochemistry-Paraffin: MCAT Antibody (2F2) [H00027349-M01]

Immunohistochemistry-Paraffin: MCAT Antibody (2F2) [H00027349-M01]

Immunohistochemistry-Paraffin: MCAT Antibody (2F2) [H00027349-M01] - Analysis of monoclonal antibody to MT on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.
Sandwich ELISA: MCAT Antibody (2F2) [H00027349-M01] - Detection limit for recombinant GST tagged MT is approximately 1ng/ml as a capture antibody.

Applications for MCAT Antibody (2F2)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MCAT

The protein encoded by this gene is found exclusively in the mitochondrion, where it catalyzes the transfer of a malonyl group from malonyl-CoA to the mitochondrial acyl carrier protein. The encoded protein may be part of a fatty acid synthase complex that is more like the type II prokaryotic and plastid complexes rather than the type I human cytosolic complex. Two transcript variants encoding different isoforms have been found for this gene.

Alternate Names

EC 2.3.1.39, fabD, FASN2C, malonyl CoA:ACP acyltransferase (mitochondrial), malonyl-CoA:acyl carrier protein transacylase, mitochondrial, malonyl-CoA-acyl carrier protein transacylase, mitochondrial, MCT[Acyl-carrier-protein] malonyltransferase, Mitochondrial malonyltransferase, MTMGC47838, NET62

Entrez Gene IDs

27349 (Human)

Gene Symbol

MCAT

UniProt

NP_775738 (Human)

Additional MCAT Products

Product Documents for MCAT Antibody (2F2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MCAT Antibody (2F2)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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