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MCF2L Antibody (1D11)

Novus Biologicals, part of Bio-Techne | Catalog # H00023263-M01

Novus Biologicals, part of Bio-Techne
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H00023263-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1D11

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

MCF2L (AAH20208, 220 a.a. ~ 319 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GTELAETELPNDVQSTSSVLCAHTEKKDKAKEDLRLALKEGHSVLESLRELQAEGSEPSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCL

Reactivity Notes

Human. Other species not tested.

Specificity

MCF2L - MCF.2 cell line derived transforming sequence-like

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for MCF2L Antibody (1D11)

Immunohistochemistry-Paraffin: MCF2L Antibody (1D11) [H00023263-M01]

Immunohistochemistry-Paraffin: MCF2L Antibody (1D11) [H00023263-M01]

Immunohistochemistry-Paraffin: MCF2L Antibody (1D11) [H00023263-M01] - Analysis of monoclonal antibody to MCF2L on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 1 ug/ml.
Sandwich ELISA: MCF2L Antibody (1D11) [H00023263-M01] - Detection limit for recombinant GST tagged MCF2L is approximately 0.03ng/ml as a capture antibody.

Applications for MCF2L Antibody (1D11)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MCF2L

Guanine nucleotide exchange factor that potentially links pathways that signal through RAC1, RHOA and CDC42. Catalyzes guanine nucleotide exchange on RHOA and CDC42 and interacts specifically with the GTP-bound form of RAC1, suggesting that it functions as an effector of RAC1. May also participate in axonal transport in the brain. Becomes activated and highly tumorigenic by truncation of the N-terminus . Isoform 5 activates CDC42

Alternate Names

DBL's big sister, DBS, FLJ12122, guanine nucleotide exchange factor DBS, KIAA0362ARHGEF14, MCF.2 cell line derived transforming sequence-like, MCF2 transforming sequence-like protein, MCF2-transforming sequence-like protein, OST

Entrez Gene IDs

23263 (Human)

Gene Symbol

MCF2L

Additional MCF2L Products

Product Documents for MCF2L Antibody (1D11)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MCF2L Antibody (1D11)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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