mCherry Antibody (857)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-43769
Key Product Details
Species Reactivity
Non-species specific
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Whole-Mount, Single Cell Western, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 857
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Full length mCherry recombinant protein
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for mCherry Antibody (857)
![Western Blot: mCherry Antibody (857) [NBP2-43769]](https://resources.bio-techne.com/images/products/mCherry-Antibody-857-Western-Blot-NBP2-43769-img0001.jpg "Western Blot: mCherry Antibody (857) [NBP2-43769]")
Western Blot: mCherry Antibody (857) [NBP2-43769]
Western Blot: mCherry Antibody (857) [NBP2-43769] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug whole cell lysate/extract of mCherry-tagged protein expressing 293T cells 5 % SDS-PAGE mCherry antibody [857] dilution: 1:1000.Applications for mCherry Antibody (857)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Assay-dependent dilution
Immunohistochemistry
Assay dependent
Immunohistochemistry Whole-Mount
Assaydependent dilution
Single Cell Western
100 ug/ml
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: mCherry
mCherry can be used as a long-wavelength hetero-FRET (fluorescence resonance energy transfer) acceptor and probe for homoFRET experiments given its high peak molar absorptivity, folding efficiency, and superior spectral properties (4). Additionally, because mCherry does not interfere with other plasmids or alter the growth of Legionella species during intracellular growth, it can be used for constitutive gene expression in a variety of gram-negative bacterial species (5). For example, a plasmid developed to constitutively express mCherry under the Ptac promoter has been used in several Legionella species including L. pneumophila, the causative agent of Legionnaires' disease (5).
References
1. Shaner, N. C., Steinbach, P. A., & Tsien, R. Y. (2005). A guide to choosing fluorescent proteins. Nature Methods, 2(12), 905-909. doi:10.1038/nmeth819
2. Bevis, B. J., & Glick, B. S. (2002). Rapidly maturing variants of the Discosoma red fluorescent protein (DsRed). Nature Biotechnology, 20(1), 83-87. https://doi.org/10.1038/nbt0102-83
3. Wall, M. A., Socolich, M., & Ranganathan, R. (2000). The structural basis for red fluorescence in the tetrameric GFP homolog DsRed. Nature Structural Biology, 7(12), 1133-1138. https://doi.org/10.1038/81992
4. Akrap, N., Seidel, T., & Barisas, B. G. (2010). Forster distances for fluorescence resonant energy transfer between mCherry and other visible fluorescent proteins. Analytical Biochemistry, 402(1), 105-106. https://doi.org/10.1016/j.ab.2010.03.026
5. Gebhardt, M. J., Jacobson, R. K., & Shuman, H. A. (2017). Seeing red; the development of pON.mCherry, a broad-host range constitutive expression plasmid for Gram-negative bacteria. Plos One, 12(3), e0173116. https://doi.org/10.1371/journal.pone.0173116
Long Name
mCherry
Alternate Names
DSRED, red fluorescent protein mCherry, Red Fluoroscent Protein
Additional mCherry Products
Product Documents for mCherry Antibody (857)
Product Specific Notices for mCherry Antibody (857)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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