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Mcl-1 Antibody (8C6) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2194

Novus Biologicals, part of Bio-Techne
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NB100-2194-0.025ml
NB100-2194

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 8C6

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Purified recombinant fragment of human MCL-1 expressed in E. Coli.

Localization

Cell Membrane, Cytoplasmic, Nuclear

Specificity

MCL-1 (8C6D4B1)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Mcl-1 Antibody (8C6) - BSA Free

Western Blot: Mcl-1 Antibody (8C6)BSA Free [NB100-2194]

Western Blot: Mcl-1 Antibody (8C6)BSA Free [NB100-2194]

Western Blot: Mcl-1 Antibody (8C6D4B1) [NB100-2194] - Analysis using MCL1 mouse mAb against Hela (1), BCBL-1 (2), Jurkat (3) and HL60 (4) cell lysate.
Immunocytochemistry/ Immunofluorescence: Mcl-1 Antibody (8C6) - BSA Free [NB100-2194]

Immunocytochemistry/ Immunofluorescence: Mcl-1 Antibody (8C6) - BSA Free [NB100-2194]

Immunocytochemistry/Immunofluorescence: Mcl-1 Antibody (8C6D4B1) [NB100-2194] - Analysis of HepG2 cells using MCL1 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry-Paraffin: Mcl-1 Antibody (8C6) - BSA Free [NB100-2194]

Immunohistochemistry-Paraffin: Mcl-1 Antibody (8C6) - BSA Free [NB100-2194]

Immunohistochemistry-Paraffin: Mcl-1 Antibody (8C6D4B1) [NB100-2194] - Analysis of human lymphnode tissues using MCL1 mouse mAb with DAB staining.

Applications for Mcl-1 Antibody (8C6) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500-1:2000
Application Notes
This antibody is useful for ELISA, Western Blo, Immunohistochemistry-Paraffin and Immunohistochemistry-Frozen.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Mcl-1

Mcl-1 (Myeloid cell leukemia-1) is Bcl-2-related and was identified as an early-induction gene that increased in expression during the differentiation of human myeloblastic leukemia cell ML-1, or exposure to different DNA damaging agents. The level of Mcl-1 is decreased in peripheral B lymphocytes undergoing apoptosis following treatment with apoptotic stimuli such as TGF-alpha 1 and forskolin. Expression of Mcl-1 is able to delay apoptosis induced by over-expression of c-myc in CHO 5AHSmyc cells. In hematopoietic FDC-P1 cells, Mcl-1 interacts with another Bcl-2-related protein, Bax, and prolongs cell viability after treatment with different apoptotic reagents.This monoclonal antibody detected a 37kd MCL1 in BCBL-1 cell lysate.

Long Name

Myeloid Cell Leukemia Sequence 1

Alternate Names

BCL2L3, Mcl1

Entrez Gene IDs

4170 (Human)

Gene Symbol

MCL1

Additional Mcl-1 Products

Product Documents for Mcl-1 Antibody (8C6) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Mcl-1 Antibody (8C6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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