MCM10 Antibody

Western Blot: MCM10 Antibody [NB100-253]

Western Blot: MCM10 Antibody [NB100-253] - No relocalization or post-translational modifications of some components of the replication machinery after UVA radiation. Asynchronous MRC5Vi cells or synchronized in early S-phase (condition S0R, see Material and Methods) are untreated or exposed to 80 and 160 kJ/m2 of UVA radiation. Subcellular localization of Cdc6, Cdc7, Dbf4, Cdc45, Mcm2, Mcm10, Orc2, PCNA, RPA32 in asynchronous cells (Asyncro.) or cells synchronized in early S-phase (Synchro.). Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0140645), licensed under a CC-BY license.

Western Blot: MCM10 Antibody [NB100-253]

Western Blot: MCM10 Antibody [NB100-253] - Detection of human MCM10 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCM10 antibody NB100-253 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Western Blot: MCM10 Antibody [NB100-253]

Western Blot: MCM10 Antibody [NB100-253] - Asynchronous MRC5Vi cells or synchronized in early S-phase (condition S0R, see Material and Methods) are untreated or exposed to 80 and 160 kJ/m2 of UVA radiation. The fractions of chromatin-bound proteins and total soluble proteins were recovered at various time points post UVA. The expression levels of Cdc6, Cdc7, Dbf4, Cdc45, Mcm2, p-Mcm2(Ser40/41), Mcm10, Orc2, PCNA, and RPA32 were detected by Western blotting in denaturing and reducing conditions. Lamin A/C and GAPDH were used as loading control for the chromatin-bound and soluble fractions, respectively. The apparent molecular weight of each protein is indicated on the right side of each blot (panel 7A). The stars (*) indicate the position of the non specific bands detected by Orc2 antibody. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0140645), licensed under a CC-BY license.