MCM2 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-288
![Western Blot: MCM2 Antibody [NB100-288]](https://resources.bio-techne.com/images/products/MCM2-Antibody-Western-Blot-NB100-288-img0018.jpg "Western Blot: MCM2 Antibody [NB100-288]")
Conjugate
Catalog #
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
IF/IHC, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
A 37-amino acid synthetic peptide, which represented a portion of human Minichromosome Maintenance Deficient 2 encoded within exon 2 (LocusLink ID 4171).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for MCM2 Antibody - BSA Free
Western Blot: MCM2 Antibody [NB100-288]
Western Blot: MCM2 Antibody [NB100-288] - Detection of human and mouse MCM2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCM2 antibody NB100-288 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.![Immunohistochemistry-Paraffin: MCM2 Antibody [NB100-288]](https://resources.bio-techne.com/images/products/MCM2-Antibody-Immunohistochemistry-NB100-288-img0017.jpg "Immunohistochemistry-Paraffin: MCM2 Antibody [NB100-288]")
Immunohistochemistry-Paraffin: MCM2 Antibody [NB100-288]
Immunohistochemistry-Paraffin: MCM2 Antibody [NB100-288] - Sample: FFPE section of human small cell lung cancer. Antibody: Affinity purified rabbit anti-MCM2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB![Immunoprecipitation: MCM2 Antibody [NB100-288]](https://resources.bio-techne.com/images/products/MCM2-Antibody-Immunoprecipitation-NB100-288-img0019.jpg "Immunoprecipitation: MCM2 Antibody [NB100-288]")
Immunoprecipitation: MCM2 Antibody [NB100-288]
Immunoprecipitation: MCM2 Antibody [NB100-288] - Detection of human MCM2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MCM2 antibody NB100-288 used for IP at 3 ug per reaction. MCM2 was also immunoprecipitated by rabbit anti-Phospho MCM2 (S108) antibody NB100-639. For blotting immunoprecipitated MCM2, NB100-288 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.Applications for MCM2 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:2000-1:10000
Immunohistochemistry-Paraffin
1:2000-1:10000
Immunoprecipitation
2-5 ug//mg lysate
Western Blot
1:1000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Reviewed Applications
Read 2 reviews rated 4 using NB100-288 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: MCM2
Long Name
Minichromosome Maintenance Protein 2
Alternate Names
BM28, CCNL1, cdc19, CDCL1, D3S3194, Mitotin
Entrez Gene IDs
4171 (Human)
Gene Symbol
MCM2
UniProt
Additional MCM2 Products
Product Documents for MCM2 Antibody - BSA Free
Product Specific Notices for MCM2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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