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MCM3 Antibody (MCM3/8972R) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24079

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-24079

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Kappa Clone # MCM3/8972R

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Summary for MCM3 Antibody (MCM3/8972R) - Azide and BSA Free

Immunogen

Recombinant fragment (aa 650-750) of human MCM3 protein (exact sequence is proprietary)

Localization

Nucleus.

Marker

Proliferation Marker

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Description

Antibody without azide - store at -20 to -80C.

Scientific Data Images for MCM3 Antibody (MCM3/8972R) - Azide and BSA Free

Immunohistochemistry-Paraffin: MCM3 Antibody (MCM3/8972R) - Azide and BSA Free [NBP3-24079] -

Formalin-fixed, paraffin-embedded human tonsil stained with MCM3 antibody (MCM3/8972R) - Azide and BSA Free. Inset: PBS instead of primary antibody, secondary only control.

Applications for MCM3 Antibody (MCM3/8972R) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2ug/ml
Application Notes
Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: MCM3

MCM3 is encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression.

Alternate Names

cervical cancer proto-oncogene 5, DNA polymerase alpha holoenzyme-associated protein P1, DNA replication factor MCM3, DNA replication licensing factor MCM3, EC 3.6.4.12, HCC5, hRlf beta subunit, MCM3 minichromosome maintenance deficient 3, MCM3 minichromosome maintenance deficient 3 (S. cerevisiae), MGC1157, minichromosome maintenance complex component 3, minichromosome maintenance deficient (S. cerevisiae) 3, minichromosome maintenance deficient 3, P1.h, p102, P1-MCM3, replication licensing factor, beta subunit, RLF subunit beta, RLFB

Gene Symbol

MCM3

Additional MCM3 Products

Product Documents for MCM3 Antibody (MCM3/8972R) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MCM3 Antibody (MCM3/8972R) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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