Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

1.0 mg/ml

View all MCM3 Antibodies »

Product Summary for MCM3 Antibody

Immunogen

The immunogen recognized by this antibody maps to a region between residues 500 and 550 of human Minichromosome Maintenance 3 using the numbering given in entry NP_002379.2 (GeneID 4172).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for MCM3 Antibody

Western Blot: MCM3 Antibody [NB100-249]

Western Blot: MCM3 Antibody [NB100-249]

Western Blot: MCM3 Antibody [NB100-249] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-MCM3 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry: MCM3 Antibody [NB100-249]

Immunohistochemistry: MCM3 Antibody [NB100-249]

Immunohistochemistry: MCM3 Antibody [NB100-249] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified goat anti-MCM3 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Western Blot: MCM3 Antibody [NB100-249]

Western Blot: MCM3 Antibody [NB100-249]

Western Blot: MCM3 Antibody [NB100-249] - Whole cell lysate (60 ug) from HeLa cells. Antibody used at 0.25 ug/ml.

Applications for MCM3 Antibody

Application
Recommended Usage

Western Blot

1:2000-1:10000
Application Notes
By Western blot, this antibody recognizes bands around 110 kDa representing MCM3.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: MCM3

The MCM (Mini-Chromosome Maintenance) complex is a key component of the pre-replication complex involved in replication licensing which restricts DNA replication to only once per cell cycle. The MCM complex is a heterohexamer of MCM2, MCM3, MCM4, MCM5, MCM6, and MCM7. MCM3 has been shown to interact with MCM5, CDC46, and the acetyltransferase MCM3AP. Alternate names for MCM3 include DNA replication licensing factor MCM3, DNA polymerase alpha holoenzyme-associated protein P1, RLF subunit beta, P102, P1-MCM3, HCC5, RLFB, and MGC1157.

Alternate Names

cervical cancer proto-oncogene 5, DNA polymerase alpha holoenzyme-associated protein P1, DNA replication factor MCM3, DNA replication licensing factor MCM3, EC 3.6.4.12, HCC5, hRlf beta subunit, MCM3 minichromosome maintenance deficient 3, MCM3 minichromosome maintenance deficient 3 (S. cerevisiae), MGC1157, minichromosome maintenance complex component 3, minichromosome maintenance deficient (S. cerevisiae) 3, minichromosome maintenance deficient 3, P1.h, p102, P1-MCM3, replication licensing factor, beta subunit, RLF subunit beta, RLFB

Entrez Gene IDs

4172 (Human)

Gene Symbol

MCM3

UniProt

NP_002379.2 (Human)

Additional MCM3 Products

Product Documents for MCM3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MCM3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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