MCM4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-33105

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP1-33105
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse

Applications

Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MCM4 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human MCM4. The exact sequence is proprietary.

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MCM4 Antibody

Western Blot: MCM4 Antibody [NBP1-33105]

Western Blot: MCM4 Antibody [NBP1-33105]

Western Blot: MCM4 Antibody [NBP1-33105] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM4 antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: MCM4 Antibody [NBP1-33105]

Immunocytochemistry/ Immunofluorescence: MCM4 Antibody [NBP1-33105]

Immunocytochemistry/Immunofluorescence: MCM4 Antibody [NBP1-33105] - Paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: MCM4 Antibody [NBP1-33105]

Immunohistochemistry-Paraffin: MCM4 Antibody [NBP1-33105]

Immunohistochemistry-Paraffin: MCM4 Antibody [NBP1-33105] - Paraffin-embedded A549 Xenograft, using antibody at 1:100 dilution.
View 5 more images

Applications for MCM4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MCM4

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported.

Alternate Names

CDC21, CDC21 homolog, CDC54, DNA replication licensing factor MCM4, EC 3.6.4.12, hCdc21, homolog of S. pombe cell devision cycle 21, MCM4 minichromosome maintenance deficient 4, MCM4 minichromosome maintenance deficient 4 (S. cerevisiae), MGC33310, minichromosome maintenance complex component 4, minichromosome maintenance deficient 4, P1-Cdc21

Gene Symbol

MCM4

Additional MCM4 Products

Product Documents for MCM4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MCM4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.