MCM4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85728

Novus Biologicals, part of Bio-Techne
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NBP1-85728
NBP1-85728-25ul
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MCM4 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LPHTLLSRFDLIFLMLDPQDEAYDRRLAHHLVALYYQSEEQAEEELLDMAVLKDYIAYAHSTIMPRLSEEASQALIEAYVDMRKIGSSRGMVSAYPRQLESLIRLAEAHAKVRLSNKVEAIDVEEAKRLHREALKQSAT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MCM4 Antibody

Immunohistochemistry-Paraffin: MCM4 Antibody [NBP1-85728]

Immunohistochemistry-Paraffin: MCM4 Antibody [NBP1-85728]

Immunohistochemistry-Paraffin: MCM4 Antibody [NBP1-85728] - Analysis in human testis and pancreas tissues. Corresponding MCM4 RNA-seq data are presented for the same tissues.
Western Blot: MCM4 Antibody [NBP1-85728]

Western Blot: MCM4 Antibody [NBP1-85728]

Western Blot: MCM4 Antibody [NBP1-85728] - Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: MCM4 Antibody [NBP1-85728]

Immunocytochemistry/ Immunofluorescence: MCM4 Antibody [NBP1-85728]

Immunocytochemistry/Immunofluorescence: MCM4 Antibody [NBP1-85728] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.
View 4 more images

Applications for MCM4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: PFA/Triton X-100

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MCM4

MCM4 is encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported.

Alternate Names

CDC21, CDC21 homolog, CDC54, DNA replication licensing factor MCM4, EC 3.6.4.12, hCdc21, homolog of S. pombe cell devision cycle 21, MCM4 minichromosome maintenance deficient 4, MCM4 minichromosome maintenance deficient 4 (S. cerevisiae), MGC33310, minichromosome maintenance complex component 4, minichromosome maintenance deficient 4, P1-Cdc21

Gene Symbol

MCM4

Additional MCM4 Products

Product Documents for MCM4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MCM4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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