MCT2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-877

Novus Biologicals, part of Bio-Techne
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NB300-877
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all MCT2 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-SNAQSVTSERETNI corresponding to C-Terminus according to NP_004722.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for MCT2 Antibody

Western Blot: MCT2 Antibody [NB300-877]

Western Blot: MCT2 Antibody [NB300-877]

Western Blot: MCT2 Antibody [NB300-877] - (1ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: MCT2 Antibody [NB300-877]

Immunohistochemistry-Paraffin: MCT2 Antibody [NB300-877]

Immunohistochemistry-Paraffin: MCT2 Antibody [NB300-877] - Staining of Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for MCT2 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

2.5 ug/ml

Peptide ELISA

Detection limit 1:128000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 40-45 kDa band observed in A549, lung epithelial (lung carcinoma) lysates (calculated MW of 52.2 kDa band according to NP_004722).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MCT2

Lactic acid and pyruvate transport across plasma membranes is catalyzed by members of the proton-linked monocarboxylate transporter (MCT) family, which has been designated solute carrier family 16. Each MCT appears to have slightly different substrate and inhibitor specificities and transport kinetics, which are related to the metabolic requirements of the tissues in which it is found. MCT2 is a high affinity transporter that catalyzes the rapid transport of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate across the plasma membrane.

Long Name

Monocarboxylate transporter 2

Alternate Names

MCT 2, SLC16A7

Entrez Gene IDs

9194 (Human)

Gene Symbol

SLC16A7

UniProt

NP_004722. (Human)

Additional MCT2 Products

Product Documents for MCT2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MCT2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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