MDA5 Antibody (2N8Z5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16132

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16132-100ul
NBP3-16132-20ul
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2N8Z5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all IFIH1/MDA5 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 900-1000 of human MDA5 (Q9BYX4). PSLITFLCKNCSVLACSGEDIHVIEKMHHVNMTPEFKELYIVRENKALQKKCADYQINGEIICKCGQAWGTMMVHKGLDLPCLKIRNFVVVFKNNSTKKQY

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MDA5 Antibody (2N8Z5)

Western Blot: MDA5 Antibody (2N8Z5) [NBP3-16132]

Western Blot: MDA5 Antibody (2N8Z5) [NBP3-16132]

Western Blot: MDA5 Antibody (2N8Z5) [NBP3-16132] - Analysis of extracts of various cell lines, using MDA5 antibody (NBP3-16132) at 1:1000 dilution.C6 cells were treated by LPS for 6 hours and Brefeldin A for 3 hours of stimulation. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Western Blot: MDA5 Antibody (2N8Z5) [NBP3-16132]

Western Blot: MDA5 Antibody (2N8Z5) [NBP3-16132]

Western Blot: MDA5 Antibody (2N8Z5) [NBP3-16132] - Analysis of extracts of BxPC-3 cells, using MDA5 antibody (NBP3-16132) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Applications for MDA5 Antibody (2N8Z5)

Application
Recommended Usage

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IFIH1/MDA5

Gene ID: 64135 DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein that is upregulated in response to treatment with beta-interferon (IFNB) and a protein kinase C-activating compound, mezerein (MEZ). Irreversible reprogramming of melanomas can be achieved by treatment with both these agents; treatment with either agent alone only achieves reversible differentiation.

Long Name

Melanoma Differentiation-Associated Protein 5

Alternate Names

AGS7, Helicard, Hlcd, IDDM19, IFIH1, IMD95, MDA-5, RH116, SGMRT1

Gene Symbol

IFIH1

Additional IFIH1/MDA5 Products

Product Documents for MDA5 Antibody (2N8Z5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MDA5 Antibody (2N8Z5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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