MDA5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-17243

Novus Biologicals, part of Bio-Techne
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NBP2-17243
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all IFIH1/MDA5 Antibodies »

Product Summary for MDA5 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human MDA5. The exact sequence is proprietary.

Localization

Cytoplasm; Nucleus (Potential)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

117 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MDA5 Antibody

Immunohistochemistry-Paraffin: MDA5 Antibody [NBP2-17243]

Immunohistochemistry-Paraffin: MDA5 Antibody [NBP2-17243]

Immunohistochemistry-Paraffin: MDA5 Antibody [NBP2-17243] - Human breast carcinoma.
MDA5 Antibody

Western Blot: MDA5 Antibody [NBP2-17243] -

Western Blot: MDA5 Antibody [NBP2-17243] - Untreated (-) and treated (+) THP-1 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MDA5 antibody [N2C1], Internal (NBP2-17243) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for MDA5 Antibody

Application
Recommended Usage

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: IFIH1/MDA5

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein that is upregulated in response to treatment with beta-interferon (IFNB) and a protein kinase C-activating compound, mezerein (MEZ). Irreversible reprogramming of melanomas can be achieved by treatment with both these agents; treatment with either agent alone only achieves reversible differentiation. [provided by RefSeq]

Long Name

Melanoma Differentiation-Associated Protein 5

Alternate Names

AGS7, Helicard, Hlcd, IDDM19, IFIH1, IMD95, MDA-5, RH116, SGMRT1

Gene Symbol

IFIH1

UniProt

Q9BYX4 (Human)

Additional IFIH1/MDA5 Products

Product Documents for MDA5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MDA5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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