Key Product Details

Species Reactivity

Validated:

Human, Mouse, Bovine, Non-human Primate

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # P2B11

Concentration

1 mg/ml

View all MDC1 Antibodies »

Product Specifications

Immunogen

GST-tagged recombinant protein corresponding to mouse MDC1 at and around the N-terminus

Localization

Nucleus

Specificity

Detects approx 184kDa. This antibody recognizes MDC1 at and around the N-terminus.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for MDC1 Antibody (P2B11)

Western Blot: MDC1 Antibody (P2B11) [NBP2-12890]

Western Blot: MDC1 Antibody (P2B11) [NBP2-12890]

Western Blot: MDC1 Antibody (P2B11) [NBP2-12890] - Western Blot analysis of Mouse Cortex and Cerebellum showing detection of 184 kDa MDC1 protein using Mouse Anti-MDC1 Monoclonal Antibody, Clone P2B11 (NBP2-12890). Lane 1: MW ladder. Lane 2: Mouse Cortex. Lane 3: Mouse Cerebellum. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-MDC1 Monoclonal Antibody (NBP2-12890) at 1:1000 for 2 hours RT. Secondary Antibody: Goat Anti-Mouse at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 184 kDa.
Immunocytochemistry/ Immunofluorescence: MDC1 Antibody (P2B11) [NBP2-12890]

Immunocytochemistry/ Immunofluorescence: MDC1 Antibody (P2B11) [NBP2-12890]

Immunocytochemistry/Immunofluorescence: MDC1 Antibody (P2B11) [NBP2-12890] - Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-MDC1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus. Magnification: 60X.
Western Blot: MDC1 Antibody (P2B11) [NBP2-12890]

Western Blot: MDC1 Antibody (P2B11) [NBP2-12890]

Western Blot: MDC1 Antibody (P2B11) [NBP2-12890] - analysis of Human 293Trap cell lysates showing detection of 184 kDa MDC1 protein using Mouse Anti-MDC1 Monoclonal Antibody, Clone P2B11 . Lane 1: MW ladder. Lane 2: 293Trap cell lysates. Load: 30 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-MDC1 Monoclonal Antibody at 1:1000 for 2 hours RT. Secondary Antibody: Goat Anti-Mouse HRP:IgG at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 184 kDa.

Applications for MDC1 Antibody (P2B11)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:2000
Application Notes
0.5 ug/ml of MDC1 Antibody was sufficient for detection of MDC1 in 10 ug of HeLa cell lysate by ECL immunoblot analysis.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MDC1

0.5 ug/mL was sufficient for detection of MDC1 in 10ug of HeLa cell lysate by ECL immunoblot analysis.

Long Name

Mediator of DNA-damage checkpoint 1

Alternate Names

NFBD1

Gene Symbol

MDC1

Additional MDC1 Products

Product Documents for MDC1 Antibody (P2B11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MDC1 Antibody (P2B11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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