MDC1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84860

Novus Biologicals, part of Bio-Techne
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NBP1-84860
NBP1-84860-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MDC1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: REAVLKDHTKIRALVRAHSEKDQPPFGDSDDSVEADKSSPGIHLERSQASTTVDINTQVEKEVPPGSAIIHIKKHQVSVEGTNQTDVKAVGGPAKLLVVSLEEAWPLHGDCETDAEEGTSLTASVVADVRKSQLPAEGDAG

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 23833192).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MDC1 Antibody

Immunocytochemistry/ Immunofluorescence: MDC1 Antibody [NBP1-84860]

Immunocytochemistry/ Immunofluorescence: MDC1 Antibody [NBP1-84860]

Immunocytochemistry/Immunofluorescence: MDC1 Antibody [NBP1-84860] - Staining of human cell line U-251 MG shows localization to nucleoplasm, nuclear bodies & focal adhesion sites.
Immunohistochemistry-Paraffin: MDC1 Antibody [NBP1-84860]

Immunohistochemistry-Paraffin: MDC1 Antibody [NBP1-84860]

Immunohistochemistry-Paraffin: MDC1 Antibody [NBP1-84860] - Staining of human liver shows low expression as expected.
Immunohistochemistry-Paraffin: MDC1 Antibody [NBP1-84860]

Immunohistochemistry-Paraffin: MDC1 Antibody [NBP1-84860]

Immunohistochemistry-Paraffin: MDC1 Antibody [NBP1-84860] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
View 1 more images

Applications for MDC1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
ICC/IF reported in scientific literature (PMID: 23833192). For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MDC1

MDC1 works with H2AX to promote recruitment of repair proteins to the sites of DNA breaks and controls damage-induced cell-cycle arrest checkpoints. Cells that lack MDC1 are sensitive to ionizing radiation.

Long Name

Mediator of DNA-damage checkpoint 1

Alternate Names

NFBD1

Entrez Gene IDs

9656 (Human)

Gene Symbol

MDC1

UniProt

Q14676

Additional MDC1 Products

Product Documents for MDC1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MDC1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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