MDM2/HDM2 Antibody (SMP14) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-2736
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse, Rat
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SMP14
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide C-SRPSTSSRRRAISE, corresponding to amino acids 154-167 of human MDM2/HDM2. [UniProt# Q00987]
Epitope
Amino acids 154 - 167.
Reactivity Notes
Rat reactivity reported in scientific literature (PMID: 15625077). Mouse reactivity reported in scientific literature (PMID: 25981963).
Localization
Nuclear and cytoplasmic (expressed predominantly in the nucleoplasm).
Specificity
The SMP14 monoclonal antibody also recognises a peptide epitope around Thr-216 of murine MDM2 (when Thr-216 is unphosphorylated) see Zhang & Prives, 2001 (PMID: 11359766) for further details. SMP14 also cross reacts with some cytokeratins (6, 14 & 16). This is only a problem when working with certain epithelial cells and not fibroblasts.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for MDM2/HDM2 Antibody (SMP14) - BSA Free
Western Blot: MDM2/HDM2 Antibody (SMP14)BSA Free [NB100-2736]
Western Blot: MDM2/HDM2 Antibody (SMP14) [NB100-2736] - Daudi whole cell protein was separated by SDS-PAGE on a 7.5% gel and transferred to PVDF membrane. The membrane was probed with anti-MDM2 antibody at 2 ug/ml and detected with an anti-mouse HRP secondary antibody using chemiluminescence.Immunohistochemistry-Paraffin: MDM2/HDM2 Antibody (SMP14) - BSA Free [NB100-2736]
Immunohistochemistry-Paraffin: MDM2/HDM2 Antibody (SMP14) [NB100-2736] - Analysis of tissue section of human breast cancer xenograft using MDM2/HDM2 antibody (clone SMP14) at 1:100 dilution. Several of the cancer cells developed a strong nuclear with weak cytoplasmic immunostaining of MDM2.Immunohistochemistry-Paraffin: MDM2/HDM2 Antibody (SMP14) - BSA Free [NB100-2736]
Immunohistochemistry-Paraffin: MDM2/HDM2 Antibody (SMP14) [NB100-2736] - Analysis of tissue section of human breast cancer xenograft using MDM2/HDM2 antibody (clone SMP14) at 1:100 dilution. Several of the cancer cells developed a strong nuclear with weak cytoplasmic immunostaining of MDM2.Applications for MDM2/HDM2 Antibody (SMP14) - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:100-1:500
Immunohistochemistry-Frozen
1:200-1:500
Immunohistochemistry-Paraffin
1:100-1:500
Immunoprecipitation
1:10-1:500
Western Blot
1:500
Application Notes
This antibody has also been reported useful in IHC on resin tissue sections. Staining of IHC-P tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Reviewed Applications
Read 1 review rated 4 using NB100-2736 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MDM2/HDM2
Long Name
Double Minute 2 Protein
Alternate Names
HDM2
Entrez Gene IDs
4193 (Human)
Gene Symbol
MDM2
UniProt
Additional MDM2/HDM2 Products
Product Documents for MDM2/HDM2 Antibody (SMP14) - BSA Free
Product Specific Notices for MDM2/HDM2 Antibody (SMP14) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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