MeCP2 Antibody (4F11)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05541

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4F11

Concentration

1 mg/ml

View all MeCP2 Antibodies »

Product Summary for MeCP2 Antibody (4F11)

Immunogen

Full length human recombinant MeCP2 expressed in and purified from E. coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for MeCP2 Antibody (4F11)

Western Blot: MeCP2 Antibody [NBP3-05541]

Western Blot: MeCP2 Antibody [NBP3-05541]

Western Blot: MeCP2 Antibody [NBP3-05541] - Western blot analysis of tissue lysates using MeCP2 Antibody at a dilution of 1:1,000 in green: [1] protein standard (red), [2] rat whole brain, [3] nuclear fraction of rat brain, [4] mouse whole brain, [5] nuclear fraction of mouse brain lysate. Strong band at about 75kDa in rat and slightly lower in mouse nuclear enriched fractions corresponds to the MeCP2 protein. The same blot was simultaneously probed with chicken pAB to GAP-43 which detects GAP-43 protein in all preparations with apparent molecular weight of 43kDa.
Immunocytochemistry/ Immunofluorescence: MeCP2 Antibody [NBP3-05541]

Immunocytochemistry/ Immunofluorescence: MeCP2 Antibody [NBP3-05541]

Immunocytochemistry/Immunofluorescence: MeCP2 Antibody [NBP3-05541] - Immunofluorescent analysis of rat cerebellum section stained with MeCP2 Antibody at a dilution of 1:500 in red and costained with chicken pAb to calbindin at a dilution of 1:2,000, in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 1 hour, cut to 45uM, and free-floating sections were stained with the above antibodies. The MeCP2 antibody specifically labels nuclei of neuronal cells to a variable degree, while calbindin is expressed in the dendrites and perikarya of Purkinje cells in the molecular layer of cerebellum.

Applications for MeCP2 Antibody (4F11)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000-1:5000

Immunohistochemistry

1:1000-5000

Western Blot

1:5000-10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50% PBS, 50% glycerol

Preservative

0.035% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.

Background: MeCP2

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of some cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females.

Alternate Names

AUTSX3, DKFZp686A24160, MeCp-2 protein, mental retardation, X-linked 79, methyl CpG binding protein 2 (Rett syndrome), X-linked 16

Gene Symbol

MECP2

Additional MeCP2 Products

Product Documents for MeCP2 Antibody (4F11)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MeCP2 Antibody (4F11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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