MED12 Antibody [CoraFluor™ 1]

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2357CL1

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Product Datasheet / COA / SDS

View Available Conjugates

Conjugate

Catalog #

Unconjugated

NB100-2357

Alexa Fluor 350

NB100-2357AF350

Alexa Fluor 405

NB100-2357AF405

Alexa Fluor 488

NB100-2357AF488

Alexa Fluor 532

NB100-2357AF532

Alexa Fluor 594

NB100-2357AF594

Alexa Fluor 647

NB100-2357AF647

Alexa Fluor 700

NB100-2357AF700

Alexa Fluor 750

NB100-2357AF750

Allophycocyanin

NB100-2357APC

Biotin

NB100-2357B

DyLight 350

NB100-2357UV

DyLight 405

NB100-2357V

DyLight 488

NB100-2357G

DyLight 550

NB100-2357R

DyLight 594

NB100-2357DL594

DyLight 650

NB100-2357C

DyLight 680

NB100-2357FR

DyLight 755

NB100-2357IR

FITC

NB100-2357F

HRP

NB100-2357H

Janelia Fluor 525

NB100-2357JF525

Janelia Fluor 549

NB100-2357JF549

Janelia Fluor 585

NB100-2357JF585

Janelia Fluor 635

NB100-2357JF635

Janelia Fluor 646

NB100-2357JF646

Janelia Fluor 669

NB100-2357JF669

PE

NB100-2357PE

PerCP

NB100-2357PCP

Product Summary
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Primate

Predicted:

Chimpanzee (100%). Backed by our 100% Guarantee.

Applications

Chromatin Immunoprecipitation (ChIP), Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

CoraFluor 1

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MED12 Antibodies »

Product Summary for MED12 Antibody [CoraFluor™ 1]

Immunogen

The immunogen recognized by this antibody maps to a region between residue 2150 and the C-terminus (residue 2212) of human Mediator of RNA Polymerase II Transcription, subunit 12 (NP_005111.1).

Reactivity Notes

Primate reactivity reported in scientific literature (PMID: 24147125).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

Applications for MED12 Antibody [CoraFluor™ 1]

Application

Recommended Usage

Chromatin Immunoprecipitation (ChIP)

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: MED12

The initiation of transcription is controlled in part by a large protein assembly known as the preinitiation complex. A component of this preinitiation complex is a 1.2 MDa protein aggregate called Mediator. This Mediator component binds with a CDK8 subcomplex which contains the protein encoded by this gene, mediator complex subunit 12 (MED12), along with MED13, CDK8 kinase, and cyclin C. The CDK8 subcomplex modulates Mediator-polymerase II interactions and thereby regulates transcription initiation and reinitation rates. The MED12 protein is essential for activating CDK8 kinase. Defects in this gene cause X-linked Opitz-Kaveggia syndrome, also known as FG syndrome, and Lujan-Fryns syndrome. (provided by RefSeq)

Long Name

Mediator of RNA Polymerase II Transcription Subunit 12

Alternate Names

ARC240, CAGH45, FGS1, HOPA, OKS, TNRC11, TRAP230

Gene Symbol

MED12

Additional MED12 Products

Product Documents for MED12 Antibody [CoraFluor™ 1]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for MED12 Antibody [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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