MED16 Antibody (3B10)

Novus Biologicals, part of Bio-Techne | Catalog # H00010025-M03

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3B10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MED16 Antibodies »

Product Specifications

Immunogen

THRAP5 (AAH17282, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MCDLRRPAAGGMMDLAYVCEWEKWSKSTHCPSVPLACAWSCRNLIAFTMDLRSDDQDLTRMIHILDTEHPWDLHSIPSEHHEAITCLEWDQSGSRLLSADADGQIKCWSM

Specificity

THRAP5 - thyroid hormone receptor associated protein 5 (3B10)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for MED16 Antibody (3B10)

Western Blot: MED16 Antibody (3B10) [H00010025-M03]

Western Blot: MED16 Antibody (3B10) [H00010025-M03]

Western Blot: MED16 Antibody (3B10) [H00010025-M03] - Analysis of THRAP5 expression in Hela S3 NE (Cat # L013V3).
Immunocytochemistry/ Immunofluorescence: MED16 Antibody (3B10) [H00010025-M03]

Immunocytochemistry/ Immunofluorescence: MED16 Antibody (3B10) [H00010025-M03]

Immunocytochemistry/Immunofluorescence: MED16 Antibody (3B10) [H00010025-M03] - Analysis of monoclonal antibody to THRAP5 on HeLa cell. Antibody concentration 10 ug/ml
ELISA: MED16 Antibody (3B10) [H00010025-M03]

ELISA: MED16 Antibody (3B10) [H00010025-M03]

ELISA: MED16 Antibody (3B10) [H00010025-M03] - Detection limit for recombinant GST tagged THRAP5 is approximately 3ng/ml as a capture antibody.

Applications for MED16 Antibody (3B10)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MED16

MED16 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymera

Alternate Names

DRIP92thyroid hormone receptor associated protein 5, mediator complex subunit 16THRAP5, mediator of RNA polymerase II transcription subunit 16, Thyroid hormone receptor-associated protein 5, Thyroid hormone receptor-associated protein complex 95 kDa component, thyroid hormone receptor-associated protein, 95-kD subunit, TRAP95, Vitamin D3 receptor-interacting protein complex 92 kDa component

Entrez Gene IDs

10025 (Human)

Gene Symbol

MED16

UniProt

AAH17282 (Human)

Additional MED16 Products

Product Documents for MED16 Antibody (3B10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MED16 Antibody (3B10)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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