MED16 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-04141
![Immunoprecipitation: MED16 Antibody [NBP2-04141]](https://resources.bio-techne.com/images/products/MED16-Antibody-Immunoprecipitation-NBP2-04141-img0001.jpg "Immunoprecipitation: MED16 Antibody [NBP2-04141]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (100%). Backed by our 100% Guarantee.
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 550 and 600 of human Mediator Complex Subunit 16 using the numbering given in entry NP_005472.2 (GeneID 10025).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for MED16 Antibody - BSA Free
Immunoprecipitation: MED16 Antibody [NBP2-04141]
Immunoprecipitation: MED16 Antibody [NBP2-04141] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-MED16 antibody NBP2-04141 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. MED16 was also immunoprecipitated by rabbit anti-MED16 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).Applications for MED16 Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2-10 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: MED16
Alternate Names
DRIP92thyroid hormone receptor associated protein 5, mediator complex subunit 16THRAP5, mediator of RNA polymerase II transcription subunit 16, Thyroid hormone receptor-associated protein 5, Thyroid hormone receptor-associated protein complex 95 kDa component, thyroid hormone receptor-associated protein, 95-kD subunit, TRAP95, Vitamin D3 receptor-interacting protein complex 92 kDa component
Gene Symbol
MED16
UniProt
Additional MED16 Products
Product Documents for MED16 Antibody - BSA Free
Product Specific Notices for MED16 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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