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MED16 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-17255

Novus Biologicals, part of Bio-Techne
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NBP2-17255

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (97%), Xenopus (90%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human MED16. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (90%), Zebrafish (84%).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

97 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MED16 Antibody

MED16 Antibody

Western Blot: MED16 Antibody [NBP2-17255] -

Western Blot: MED16 Antibody [NBP2-17255] - Sample (30 ug of whole cell lysate)
A: A431
B: HeLa
C: HepG2
5% SDS PAGE
NBP2-17255 diluted at 1:1000

Applications for MED16 Antibody

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MED16

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Alternate Names

DRIP92thyroid hormone receptor associated protein 5, mediator complex subunit 16THRAP5, mediator of RNA polymerase II transcription subunit 16, Thyroid hormone receptor-associated protein 5, Thyroid hormone receptor-associated protein complex 95 kDa component, thyroid hormone receptor-associated protein, 95-kD subunit, TRAP95, Vitamin D3 receptor-interacting protein complex 92 kDa component

Gene Symbol

MED16

UniProt

Additional MED16 Products

Product Documents for MED16 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MED16 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.