MED7 Antibody (PCRP-MED7-1B8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13816

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # PCRP-MED7-1B8

Concentration

0.2 mg/ml

View all MED7 Antibodies »

Product Specifications

Immunogen

Recombinant full-length human MED7 protein (Uniprot: O43513)

Localization

Nucleus, Nuclear bodies

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

27.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14071)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for MED7 Antibody (PCRP-MED7-1B8)

Immunocytochemistry/ Immunofluorescence: MED7 Antibody (PCRP-MED7-1B8) [NBP3-13816]

Immunocytochemistry/ Immunofluorescence: MED7 Antibody (PCRP-MED7-1B8) [NBP3-13816]

Immunocytochemistry/Immunofluorescence: MED7 Antibody (PCRP-MED7-1B8) [NBP3-13816] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using MED7 Antibody (PCRP-MED7-1B8) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).
Flow Cytometry: MED7 Antibody (PCRP-MED7-1B8) [NBP3-13816]

Flow Cytometry: MED7 Antibody (PCRP-MED7-1B8) [NBP3-13816]

Flow Cytometry: MED7 Antibody (PCRP-MED7-1B8) [NBP3-13816] - Flow cytometric analysis of PFA-fixed HeLa cells. MED7 antibody (PCRP-MED7-1B8) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
Protein Array: MED7 Antibody (PCRP-MED7-1B8) [NBP3-13816]

Protein Array: MED7 Antibody (PCRP-MED7-1B8) [NBP3-13816]

Protein Array: MED7 Antibody (PCRP-MED7-1B8) [NBP3-13816] - Analysis of Protein Array containing more than 19,000 full-length human proteins using MED7 Antibody (PCRP-MED7-1B8).

Applications for MED7 Antibody (PCRP-MED7-1B8)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunoprecipitation

1-2 ug/100-500 ug protein
Application Notes
ELISA: use assay dependent concentration

Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: MED7

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Two transcript variants encoding the same protein have been found for this gene.

Alternate Names

Activator-recruited cofactor 34 kDa component, Cofactor required for Sp1 transcriptional activation subunit 9, cofactor required for Sp1 transcriptional activation, subunit 9 (33kD), cofactor required for Sp1 transcriptional activation, subunit 9, 33kDa, CRSP33CRSP complex subunit 9, CRSP9hMED7, mediator complex subunit 7ARC34, mediator of RNA polymerase II transcription subunit 7, MGC12284, RNA polymerase transcriptional regulation mediator subunit 7 homolog, Transcriptional coactivator CRSP33

Gene Symbol

MED7

UniProt

O43513 (Human)

Additional MED7 Products

Product Documents for MED7 Antibody (PCRP-MED7-1B8)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MED7 Antibody (PCRP-MED7-1B8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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