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MEF2C Antibody (CL0368) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44011

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0368

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of Q06413, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for MEF2C Antibody (CL0368) - Azide and BSA Free

MEF2C Antibody (CL0368) - Azide and BSA Free Western Blot: MEF2C Antibody [NBP3-44011]

Western Blot: MEF2C Antibody [NBP3-44011]

Lane 1: Marker [kDa]
Lane 2: Negative control (vector only transfected HEK293T lysate) 
Lane 3: MEF2C Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)
MEF2C Antibody (CL0368) - Azide and BSA Free Immunohistochemistry-Paraffin: MEF2C Antibody [NBP3-44011]

Immunohistochemistry-Paraffin: MEF2C Antibody [NBP3-44011]

Staining of human cerebellum shows strong nuclear positivity in Purkinje cells.
MEF2C Antibody (CL0368) - Azide and BSA Free Immunohistochemistry-Paraffin: MEF2C Antibody [NBP3-44011]

Immunohistochemistry-Paraffin: MEF2C Antibody [NBP3-44011]

Staining of human cerebral cortex shows strong immunoreactivity in neuronal cells.

Applications for MEF2C Antibody (CL0368) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF,Fixation/Permeabilization: PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MEF2C

Transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. Controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. May also be involved in neurogenesis and in the development of cortical architecture . Isoform 3 and isoform 4, which lack the repressor domain, are more active than isoform 1 and isoform 2

Long Name

Myocyte-specific Enhancer Factor 2C

Alternate Names

DEL5q14.3

Gene Symbol

MEF2C

Additional MEF2C Products

Product Documents for MEF2C Antibody (CL0368) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MEF2C Antibody (CL0368) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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