MEF2C Antibody (CL0369)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-30671

Novus Biologicals, part of Bio-Techne
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NBP2-30671
NBP2-30671-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0369

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MEF2C Antibodies »

Product Summary for MEF2C Antibody (CL0369)

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: PPNFEMPVSIPVSSHNSLVYSNPVSSLGNPNLLPLAHPSLQRNSMSPGVTHRPPSAGNTGGLMGGDLTSGAGTSAGNGYGNPRNSPGLLVSPGNLNKNMQAKSPPPMNLGMNNRKPDLRVLIPPGSKNTMPSVNQRINN

Epitope

NLLPLAHPSL

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for MEF2C Antibody (CL0369)

Western Blot: MEF2C Antibody (CL0369) [NBP2-30671]

Western Blot: MEF2C Antibody (CL0369) [NBP2-30671]

Western Blot: MEF2C Antibody (CL0369) [NBP2-30671] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: MEF2C Antibody (CL0369) [NBP2-30671]

Immunocytochemistry/ Immunofluorescence: MEF2C Antibody (CL0369) [NBP2-30671]

Immunocytochemistry/Immunofluorescence: MEF2C Antibody (CL0369) [NBP2-30671] - Staining of RH-30 cells using the Anti-MEF2C monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry: MEF2C Antibody (CL0369) [NBP2-30671]

Immunohistochemistry: MEF2C Antibody (CL0369) [NBP2-30671]

Immunohistochemistry: MEF2C Antibody (CL0369) [NBP2-30671] - Staining of human pancreas shows absence of staining in the exocrine glandular cells (negative control).
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Applications for MEF2C Antibody (CL0369)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MEF2C

Transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. Controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. May also be involved in neurogenesis and in the development of cortical architecture . Isoform 3 and isoform 4, which lack the repressor domain, are more active than isoform 1 and isoform 2

Long Name

Myocyte-specific Enhancer Factor 2C

Alternate Names

DEL5q14.3

Gene Symbol

MEF2C

UniProt

Q06413

Additional MEF2C Products

Product Documents for MEF2C Antibody (CL0369)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MEF2C Antibody (CL0369)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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