MEF2D Antibody (PCRP-MEF2D-3A4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13967

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # PCRP-MEF2D-3A4

Concentration

0.2 mg/ml

View all MEF2D Antibodies »

Product Summary for MEF2D Antibody (PCRP-MEF2D-3A4)

Immunogen

Recombinant full-length human MEF2D protein (Uniprot: Q14814)

Reactivity Notes

Predicted to react in Mouse and Rat.

Localization

Nucleus, Translocated by HDAC4 to nuclear dots

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

55.94 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14120)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for MEF2D Antibody (PCRP-MEF2D-3A4)

Immunocytochemistry/ Immunofluorescence: MEF2D Antibody (PCRP-MEF2D-3A4) [NBP3-13967]

Immunocytochemistry/ Immunofluorescence: MEF2D Antibody (PCRP-MEF2D-3A4) [NBP3-13967]

Immunocytochemistry/Immunofluorescence: MEF2D Antibody (PCRP-MEF2D-3A4) [NBP3-13967] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using MEF2D Antibody (PCRP-MEF2D-3A4) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).
Immunohistochemistry-Paraffin: MEF2D Antibody (PCRP-MEF2D-3A4) [NBP3-13967]

Immunohistochemistry-Paraffin: MEF2D Antibody (PCRP-MEF2D-3A4) [NBP3-13967]

Immunohistochemistry-Paraffin: MEF2D Antibody (PCRP-MEF2D-3A4) [NBP3-13967] - Formalin-fixed, paraffin-embedded human rhabdomyosarcoma stained with MEF2D antibody (PCRP-MEF2D-3A4). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.
Flow Cytometry: MEF2D Antibody (PCRP-MEF2D-3A4) [NBP3-13967]

Flow Cytometry: MEF2D Antibody (PCRP-MEF2D-3A4) [NBP3-13967]

Flow Cytometry: MEF2D Antibody (PCRP-MEF2D-3A4) [NBP3-13967] - Flow cytometric analysis of PFA-fixed HeLa cells. MEF2D antibody (PCRP-MEF2D-3A4) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
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Applications for MEF2D Antibody (PCRP-MEF2D-3A4)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Immunoprecipitation

1-2 ug/100-500 ug protein
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes

Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: MEF2D

Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific, growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. Plays a critical role in the regulation of neuronal apoptosis

Alternate Names

DKFZp686I1536, MADS box transcription enhancer factor 2, polypeptide D (myocyte enhancerfactor 2D), MEF2D/DAZAP1 fusion, myocyte enhancer factor 2D, myocyte enhancer factor 2D/deleted in azoospermia associated protein 1 fusionprotein, myocyte-specific enhancer factor 2D

Gene Symbol

MEF2D

UniProt

Q14814 (Human)

Additional MEF2D Products

Product Documents for MEF2D Antibody (PCRP-MEF2D-3A4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MEF2D Antibody (PCRP-MEF2D-3A4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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