MEF2D Antibody (PCRP-MEF2D-3A4) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14120
![Protein Array: MEF2D Antibody (PCRP-MEF2D-3A4) - Azide and BSA Free [NBP3-14120]](https://resources.bio-techne.com/images/products/MEF2D-Antibody-PCRP-MEF2D-3A4-Azide-and-BSA-Free-Protein-Array-NBP3-14120-img0001.jpg "Protein Array: MEF2D Antibody (PCRP-MEF2D-3A4) - Azide and BSA Free [NBP3-14120]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Protein Array
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # PCRP-MEF2D-3A4
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for MEF2D Antibody (PCRP-MEF2D-3A4) - Azide and BSA Free
Immunogen
Recombinant full-length human MEF2D protein (Uniprot: Q14814)
Reactivity Notes
Predicted to react in Mouse and Rat.
Localization
Nucleus, Translocated by HDAC4 to nuclear dots
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
55.94 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13967).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for MEF2D Antibody (PCRP-MEF2D-3A4) - Azide and BSA Free
Protein Array: MEF2D Antibody (PCRP-MEF2D-3A4) - Azide and BSA Free [NBP3-14120]
Protein Array: MEF2D Antibody (PCRP-MEF2D-3A4) - Azide and BSA Free [NBP3-14120] - Analysis of Protein Array containing more than 19,000 full-length human proteins using MEF2D Antibody (PCRP-MEF2D-3A4).
MEF2D Antibody (PCRP-MEF2D-3A4)
Formalin-fixed, paraffin-embedded human rhabdomyosarcoma stained with MEF2D antibody (PCRP-MEF2D-3A4). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.Applications for MEF2D Antibody (PCRP-MEF2D-3A4) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Immunoprecipitation
1-2 ug per 100-500 ug protein
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: MEF2D
Alternate Names
DKFZp686I1536, MADS box transcription enhancer factor 2, polypeptide D (myocyte enhancerfactor 2D), MEF2D/DAZAP1 fusion, myocyte enhancer factor 2D, myocyte enhancer factor 2D/deleted in azoospermia associated protein 1 fusionprotein, myocyte-specific enhancer factor 2D
Gene Symbol
MEF2D
Additional MEF2D Products
Product Documents for MEF2D Antibody (PCRP-MEF2D-3A4) - Azide and BSA Free
Product Specific Notices for MEF2D Antibody (PCRP-MEF2D-3A4) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...