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MEK1/2 [p Ser221, p Ser217] Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20686

Novus Biologicals, part of Bio-Techne
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NBP3-20686

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit

Format

BSA Free

Concentration

1 mg/ml

Product Summary for MEK1/2 [p Ser221, p Ser217] Antibody - BSA Free

Immunogen

MEK1/2 (Phospho-Ser217/Ser221) antibody was raised against a peptide sequence around phosphorylation site of serine 217/221 (I-D-S (p) -M-A) derived from Human MEK1/2.

Modification

p Ser221, p Ser217

Specificity

This antibody detects endogenous level of MEK1/2 only when phosphorylated at serine 217/221.

Clonality

Polyclonal

Host

Rabbit

Scientific Data Images for MEK1/2 [p Ser221, p Ser217] Antibody - BSA Free

MEK1/2 [p Ser217, p Ser221] Antibody

Western Blot: MEK1/2 [p Ser217, p Ser221] Antibody [NBP3-20686] -

Western Blot: MEK1/2 [p Ser217, p Ser221] Antibody [NBP3-20686] - Analysis of lysed extracts from HeLa cell untreated or treated with UV MEK1/2 (Phospho-Ser217/Ser221) Antibody.
MEK1/2 [p Ser217, p Ser221] Antibody

Immunohistochemistry: MEK1/2 [p Ser217, p Ser221] Antibody [NBP3-20686] -

Immunohistochemistry: MEK1/2 [p Ser217, p Ser221] Antibody [NBP3-20686] - Analysis of paraffin-embedded human breast carcinoma tissue using MEK1/2 (Phospho-Ser217/Ser221).

Applications for MEK1/2 [p Ser221, p Ser217] Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:100

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Epitope affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 °C.

Background: MEK1/MEK2

MEK1 and MEK2, also known as MAP2K1 and MAP2K2, are activated in response to mitogenic stimuli. Activation occurs through dual phosphorylation at S218/S222 of MEK1 and S222/S226 of MEK2 by several upstream MAP3Ks. Both MEK1 and MEK2 are dual-specificity protein kinases, phosphorylating and activating ERK1 and ERK2 at Thr and Tyr positions.

Long Name

Mitogen-activated Protein Kinase Kinase 1

Alternate Names

CFC3, CFC4, dual specificity mitogen-activated protein kinase kinase 1, dual specificity mitogen-activated protein kinase kinase 2, ERK activator kinase 1, ERK activator kinase 2, MAP kinase kinase 2, MAPK/ERK kinase 1, MAPK/ERK kinase 2, MAPKK 1, MAPKK1, MAPKK2, MEK 1, MEK1, MEK2, MEL, mitogen-activated protein kinase kinase 2, p45, MKK1, MKK2, PRKMK1, PRKMK2, protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)

Gene Symbol

MAP2K1

Additional MEK1/MEK2 Products

Product Documents for MEK1/2 [p Ser221, p Ser217] Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MEK1/2 [p Ser221, p Ser217] Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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