MEK1 [p Thr292] Antibody (4E7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26413

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4E7

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MEK1 Antibodies »

Product Summary for MEK1 [p Thr292] Antibody (4E7)

Immunogen

A synthesized peptide derived from Human MEK1 [Thr292] [UniProt Q02750]

Modification

p Thr 292

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MEK1 [p Thr292] Antibody (4E7)

MEK1 [p Thr292] Antibody (4E7)

Western Blot: MEK1 [p Thr292] Antibody (4E7) [NBP3-26413] -

Western Blot: MEK1 [p Thr292] Antibody (4E7) [NBP3-26413] - Positive Western Blot detected in Hela whole cell lysate.
All lanes: MEK1 [p Thr292] Antibody at 1.645ug/ml.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 45 KDa
Observed band size: 45 KDa

Applications for MEK1 [p Thr292] Antibody (4E7)

Application
Recommended Usage

Western Blot

1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: MEK1

MEK 1 (MAP Kinase Kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation (Ahn, 1993; Chong et al., 2003). This pathway also plays a key role in synaptic plasticity in brain (Adams and Sweatt, 2002). Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase (Kyriakis et al., 1991; Seger et al., 1991; Crews et al., 1992) Conversely there also appears to be a feedback phosphorylation of MEK 1 by MAP kinase. The sites on MEK 1 that are phosphorylated by MAP kinase are Thr292 and Thr386 (Mansour et al., 1994).

Long Name

Mitogen-activated Protein Kinase Kinase 1

Alternate Names

MAP2K1, MKK1

Gene Symbol

MAP2K1

Additional MEK1 Products

Product Documents for MEK1 [p Thr292] Antibody (4E7)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MEK1 [p Thr292] Antibody (4E7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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