MEK1 Antibody (9B6)

Immunoprecipitation: MEK1 Antibody (9B6) [NBP3-26153] -

Immunoprecipitation: MEK1 Antibody (9B6) [NBP3-26153] - Immunoprecipitating MEK1 in Hela whole cell lysate.
Lane 1: Rabbit control IgG instead of NBP3-26153 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).
Lane 2: NBP3-26153 (2ug) + Hela whole cell lysate (500ug).
Lane 3: Hela whole cell lysate (10ug).

Immunohistochemistry: MEK1 Antibody (9B6) [NBP3-26153] -

Immunohistochemistry: MEK1 Antibody (9B6) [NBP3-26153] - Image of MEK1 Antibody (9B6) diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Western Blot: MEK1 Antibody (9B6) [NBP3-26153] -

Western Blot: MEK1 Antibody (9B6) [NBP3-26153] - Positive Western Blot detected in: Hela whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate, 293T whole cell lysate, A549 whole cell lysate, U251 whole cell lysate, Rat brain tissue.
All lanes: MEK1 Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 44, 41 kDa
Observed band size: 44 kDa