MEK1 [p Thr292] Antibody (SR1773)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21559

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR1773

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MEK1 Antibodies »

Product Summary for MEK1 [p Thr292] Antibody (SR1773)

Immunogen

A synthesized peptide derived from human phospho-MEK1 [Thr292] (Uniprot #: Q02750 )

Modification

p Thr 292

Specificity

Detects endogenous levels of phospho-MEK1 [Thr292]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MEK1 [p Thr292] Antibody (SR1773)

Western Blot: MEK1 [p Thr 292] Antibody (SR1773) [NBP3-21559] -

Western Blot: MEK1 [p Thr 292] Antibody (SR1773) [NBP3-21559] - Western blot analysis using NBP3-21559 in (1) HeLa cell lysate; (2) HeLa cell treated with Nocodazole.

Applications for MEK1 [p Thr292] Antibody (SR1773)

Application
Recommended Usage

Western Blot

1:500-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MEK1

MEK 1 (MAP Kinase Kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation (Ahn, 1993; Chong et al., 2003). This pathway also plays a key role in synaptic plasticity in brain (Adams and Sweatt, 2002). Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase (Kyriakis et al., 1991; Seger et al., 1991; Crews et al., 1992) Conversely there also appears to be a feedback phosphorylation of MEK 1 by MAP kinase. The sites on MEK 1 that are phosphorylated by MAP kinase are Thr292 and Thr386 (Mansour et al., 1994).

Long Name

Mitogen-activated Protein Kinase Kinase 1

Alternate Names

MAP2K1, MKK1

Gene Symbol

MAP2K1

Additional MEK1 Products

Product Documents for MEK1 [p Thr292] Antibody (SR1773)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MEK1 [p Thr292] Antibody (SR1773)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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