MEK1 [p Ser218, p Ser222] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB500-139
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Predicted:
Bovine (100%), Canine (100%), Chicken (100%), Primate (100%), Rat (100%), Xenopus (100%), Zebrafish (100%). Backed by our 100% Guarantee.
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser218/222 conjugated to KLH. Accession # Q02750
Modification
p Ser218, p Ser222
Specificity
Specific for endogenous levels of the ~45 kDa MEK1 protein phosphorylated at Ser218/222. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for MEK1 [p Ser218, p Ser222] Antibody
![Western Blot: MEK1 [p Ser218, p Ser222] Antibody [NB500-139]](https://resources.bio-techne.com/images/products/MEK1-[p-Ser218-p-Ser222]-Antibody-Western-Blot-NB500-139-img0001.jpg "Western Blot: MEK1 [p Ser218, p Ser222] Antibody [NB500-139]")
Western Blot: MEK1 [p Ser218, p Ser222] Antibody [NB500-139]
Western Blot: MEK1 [p Ser218, p Ser222] Antibody [NB500-139] - Western Blot of NIH 3T3 cell lysates showing specific immunolabeling of the ~45kDa MEK 1/2 protein phosphorylated at Ser218 and Ser222. The cells were either serum starved (Control) or incubated in the presence of serum (Serum). Immunolabeling of an additional band at ~95kDa was also observed.Applications for MEK1 [p Ser218, p Ser222] Antibody
Application
Recommended Usage
Western Blot
1:1000
Application Notes
This antibody is useful for Western blot where a band at 45 kDa is seen.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: MEK1
Long Name
Mitogen-activated Protein Kinase Kinase 1
Alternate Names
MAP2K1, MKK1
Gene Symbol
MAP2K1
UniProt
Additional MEK1 Products
Product Documents for MEK1 [p Ser218, p Ser222] Antibody
Product Specific Notices for MEK1 [p Ser218, p Ser222] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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