MEK1 [p Thr386] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB500-144
Key Product Details
Species Reactivity
Validated:
Human, Rat
Predicted:
Bovine (100%), Canine (100%), Chicken (100%), Mouse (100%), Primate (100%), Xenopus (100%). Backed by our 100% Guarantee.
Applications
Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr386 conjugated to KLH. Accession # Q02750
Reactivity Notes
Human reactivity reported in scientific literature (PMID: 26446942)
Modification
p Thr386
Specificity
Specific for endogenous levels of the ~45 kDa MEK1 protein phosphorylated at Thr386. The immunolabeling is completely eliminated by treatment with lambda-phosphatase.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for MEK1 [p Thr386] Antibody
![Western Blot: MEK1 [p Thr386] Antibody [NB500-144]](https://resources.bio-techne.com/images/products/MEK1-[p-Thr386]-Antibody-Western-Blot-NB500-144-img0001.jpg "Western Blot: MEK1 [p Thr386] Antibody [NB500-144]")
Western Blot: MEK1 [p Thr386] Antibody [NB500-144]
Western Blot: MEK1 [p Thr386] Antibody [NB500-144] - Analysis of recombinant WT and mutant MEK 1 immunolabeled with the Anti Thr386 MEK1 antibody. Lanes 1 and 2 are WT MEK 1 and Lanes 3 and 4 are mutant MEK 1 (T386A). MAP kinase was coexpressed in the samples run in Lanes 2 and 4.Applications for MEK1 [p Thr386] Antibody
Application
Recommended Usage
Simple Western
1:50
Western Blot
1:1000
Application Notes
NB 500-144 can be used in Dot blots and Western blot where a band is seen at ~45 kDa, representing MEK 1. The investigator should determine the optimal dilution for a specific application.
See Simple Western Antibody Database for Simple Western validation: tested in HeLa + EGF; separated by size, antibody dilution of 1:50.
See Simple Western Antibody Database for Simple Western validation: tested in HeLa + EGF; separated by size, antibody dilution of 1:50.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: MEK1
Long Name
Mitogen-activated Protein Kinase Kinase 1
Alternate Names
MAP2K1, MKK1
Entrez Gene IDs
5604 (Human)
Gene Symbol
MAP2K1
UniProt
Additional MEK1 Products
Product Documents for MEK1 [p Thr386] Antibody
Product Specific Notices for MEK1 [p Thr386] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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