MEK2 Antibody (OTI8G6)
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47836
![Immunohistochemistry-Paraffin: MEK2 Antibody (OTI8G6) [NBP1-47836]](https://resources.bio-techne.com/images/products/MEK2-Antibody-8G6-Immunohistochemistry-Paraffin-NBP1-47836-img0016.jpg "Immunohistochemistry-Paraffin: MEK2 Antibody (OTI8G6) [NBP1-47836]")
![Western Blot: MEK2 Antibody (OTI8G6) [NBP1-47836]](https://resources.bio-techne.com/images/products/MEK2-Antibody-8G6-Western-Blot-NBP1-47836-img0028.jpg "Western Blot: MEK2 Antibody (OTI8G6) [NBP1-47836]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI8G6
Concentration
1 mg/ml
Product Summary for MEK2 Antibody (OTI8G6)
Immunogen
Full-length protein expressed in 293T cell transfected with human MAP2K2 expression vector
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Specificity
This antibody is specific for Homo sapiens mitogen-activated protein kinase kinase 2 (MAP2K2).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
44.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for MEK2 Antibody (OTI8G6)
Western Blot: MEK2 Antibody (OTI8G6) [NBP1-47836]
Western Blot: MEK2 Antibody (8G6) [NBP1-47836] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MEK2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MEK2.![Immunohistochemistry-Paraffin: MEK2 Antibody (OTI8G6) [NBP1-47836]](https://resources.bio-techne.com/images/products/MEK2-Antibody-8G6-Immunohistochemistry-Paraffin-NBP1-47836-img0026.jpg "Immunohistochemistry-Paraffin: MEK2 Antibody (OTI8G6) [NBP1-47836]")
Immunohistochemistry-Paraffin: MEK2 Antibody (OTI8G6) [NBP1-47836]
Immunohistochemistry-Paraffin: MEK2 Antibody (8G6) [NBP1-47836] - Staining of paraffin-embedded Human lung tissue using anti-MEK2 mouse monoclonal antibody.![Flow Cytometry: MEK2 Antibody (OTI8G6) [NBP1-47836]](https://resources.bio-techne.com/images/products/MEK2-Antibody-8G6-Flow-Cytometry-NBP1-47836-img0015.jpg "Flow Cytometry: MEK2 Antibody (OTI8G6) [NBP1-47836]")
Flow Cytometry: MEK2 Antibody (OTI8G6) [NBP1-47836]
Flow Cytometry: MEK2 Antibody (8G6) [NBP1-47836] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MEK2 antibody, and then analyzed by flow cytometry.![Immunohistochemistry-Paraffin: MEK2 Antibody (OTI8G6) [NBP1-47836]](https://resources.bio-techne.com/images/products/MEK2-Antibody-8G6-Immunohistochemistry-Paraffin-NBP1-47836-img0017.jpg "Immunohistochemistry-Paraffin: MEK2 Antibody (OTI8G6) [NBP1-47836]")
![Immunohistochemistry-Paraffin: MEK2 Antibody (OTI8G6) [NBP1-47836]](https://resources.bio-techne.com/images/products/MEK2-Antibody-8G6-Immunohistochemistry-Paraffin-NBP1-47836-img0018.jpg "Immunohistochemistry-Paraffin: MEK2 Antibody (OTI8G6) [NBP1-47836]")
![Immunohistochemistry-Paraffin: MEK2 Antibody (OTI8G6) [NBP1-47836]](https://resources.bio-techne.com/images/products/MEK2-Antibody-8G6-Immunohistochemistry-Paraffin-NBP1-47836-img0024.jpg "Immunohistochemistry-Paraffin: MEK2 Antibody (OTI8G6) [NBP1-47836]")
![Immunohistochemistry-Paraffin: MEK2 Antibody (OTI8G6) [NBP1-47836]](https://resources.bio-techne.com/images/products/MEK2-Antibody-8G6-Immunohistochemistry-Paraffin-NBP1-47836-img0025.jpg "Immunohistochemistry-Paraffin: MEK2 Antibody (OTI8G6) [NBP1-47836]")
Applications for MEK2 Antibody (OTI8G6)
Application
Recommended Usage
Flow Cytometry
1:100
Immunohistochemistry
1:150
Immunohistochemistry-Paraffin
1:150
Western Blot
1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: MEK2
Long Name
Mitogen-activated Protein Kinase Kinase 2
Alternate Names
MAP2K2, MKK2
Entrez Gene IDs
5605 (Human)
Gene Symbol
MAP2K2
Additional MEK2 Products
Product Documents for MEK2 Antibody (OTI8G6)
Product Specific Notices for MEK2 Antibody (OTI8G6)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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