MEKK3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24979

Novus Biologicals, part of Bio-Techne
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NBP3-24979
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (94%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MEKK3 Antibodies »

Product Specifications

Immunogen

This antibody has been engineered to specifically recognize the recombinant protein MEKK3 using the following amino acid sequence: NELSILLKNQDDLDKAIDILDRSSSMKSLRILLLSQDRNHNSSSPHSGVSRQVRIKASQSAGDINTIYQPPEPRSRHLSVSSQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MEKK3 Antibody

MEKK3 Antibody Immunohistochemistry-Paraffin: MEKK3 Antibody [NBP3-24979]

Immunohistochemistry-Paraffin: MEKK3 Antibody [NBP3-24979]

Staining of human colon shows cytoplasmic positivity in glandular cells.
MEKK3 Antibody Western Blot: MEKK3 Antibody [NBP3-24979]

Western Blot: MEKK3 Antibody [NBP3-24979]

Lane 1: Marker [kDa] 250,130,95,72,55,36,28,17,10
Lane 2: RT-4
Lane 3: U-251MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Applications for MEKK3 Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 µg/ml
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MEKK3

MEKK3 is a Ser/Thr protein kinase belonging to the MAP/ERK kinase kinase family (1). Closely related to MEKK2, MEKK3 is expressed in a multitude of tissues. MEKK3 directly regulates SAPKs and ERKs pathway via SEK and MEK1, respectively but not the p38 pathway (2) and is also involved in the NF-kB pathway (3). MEKK3 as well as MEKK2 bind, phosphorylate and activate MEK5 through their PB1 domain (4). hKSR-2 (human kinase suppressor of ras-2) has been shown to regulate the activity of MEKK3 (5).

Long Name

Mitogen-activated Protein Kinase Kinase Kinase 3

Alternate Names

MAP3K3, MAPKKK3

Gene Symbol

MAP3K3

Additional MEKK3 Products

Product Documents for MEKK3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MEKK3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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