Melan-A/MART-1 Antibody (A103) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-46603

Novus Biologicals, part of Bio-Techne
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NBP2-46603
NBP2-46603SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # A103

Format

BSA Free

Concentration

1.0 mg/ml

View all Melan-A/MART-1 Antibodies »

Product Specifications

Immunogen

Recombinant human Melan-A/MART-1. [Uniprot# Q16655]

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Melan-A/MART-1 Antibody (A103) - BSA Free

Western Blot: Melan-A/MART-1 Antibody (A103)BSA Free [NBP2-46603]

Western Blot: Melan-A/MART-1 Antibody (A103)BSA Free [NBP2-46603]

Western Blot: Melan-A/MART-1 Antibody (A103) [NBP2-46603] - Total protein from human skin, pancreas and kidney and mouse kidney was separated on a 4-20% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Melan-A in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A103) - BSA Free [NBP2-46603]

Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A103) - BSA Free [NBP2-46603]

Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A103) [NBP2-46603] - Analysis of FFPE tissue section of Human Skin Cancer/Melanoma using Melan-A/MART-1 antibody (A103) at 1:100 dilution. The antibody depicted very specific staining which was cytoplasmic with strong punctate appearance in peri-nuclear regions (ER and Golgi) of most of the cancerous cells and membranous-cytoplasmic pattern in some necrotic areas.
Flow Cytometry: Melan-A/MART-1 Antibody (A103) - BSA Free [NBP2-46603]

Flow Cytometry: Melan-A/MART-1 Antibody (A103) - BSA Free [NBP2-46603]

Flow Cytometry: Melan-A/MART-1 Antibody (A103) - BSA Free [NBP2-46603] - An intracellular stain was performed on SK-MEL-28 cells with Melan-A/MART-1 Antibody (A103) NBP2-46603AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.
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Applications for Melan-A/MART-1 Antibody (A103) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 27565163)

Immunohistochemistry

1:100

Immunohistochemistry-Frozen

1:100

Immunohistochemistry-Paraffin

1:100

Western Blot

2 ug/ml

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Melan-A/MART-1

Melan-A/MART-1, also known as MLANA or melanoma antigen recognized by T-cells 1, is expressed exclusively in retina, melanoma, and melanocyte cells. Melan-A interacts with GPR143, ensuring its stability, as well as with PMEL. The interaction between Melan-A and PMEL is crucial to the development of stage II melanosomes, which are organelles that contain and store melanin. Melan-A is found in the membranes of the Golgi apparatus, endoplasmic reticulum, as well as the cell membrane itself. Melan-A's expression on the exterior of melanoma cells are key in the recognition and subsequent lysis of these cells by cytolytic T-Lymphocytes, giving Melan-A a large role in cancer research pertaining to melanomas.

Alternate Names

Antigen LB39-AA, Antigen SK29-AA, MART-1, MelanA, MLANA

Gene Symbol

MLANA

Additional Melan-A/MART-1 Products

Product Documents for Melan-A/MART-1 Antibody (A103) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Melan-A/MART-1 Antibody (A103) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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