Melan-A/MART-1 Antibody (CL12863) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44177
![Melan-A/MART-1 Antibody (CL12863) - Azide and BSA Free Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody [NBP3-44177]](https://resources.bio-techne.com/images/products/nbp3-44177_mouse-melan-a-mart-1-mab-cl12863-azide-and-bsa-free-2822025139191.jpg "Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody [NBP3-44177]")
![Melan-A/MART-1 Antibody (CL12863) - Azide and BSA Free Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody [NBP3-44177]](https://resources.bio-techne.com/images/products/nbp3-44177_mouse-melan-a-mart-1-mab-cl12863-azide-and-bsa-free-2822025128211.jpg "Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody [NBP3-44177]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL12863
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
This antibody was generated using a recombinant protein sequence of Q16655, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Melan-A/MART-1 Antibody (CL12863) - Azide and BSA Free
![Melan-A/MART-1 Antibody (CL12863) - Azide and BSA Free Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody [NBP3-44177]](https://resources.bio-techne.com/images/products/nbp3-44177_mouse-melan-a-mart-1-mab-cl12863-azide-and-bsa-free-282202512362427.jpg "Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody [NBP3-44177]")
Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody [NBP3-44177]
Staining of human cervix shows no positivity in squamous epithelial cells as expected.![Melan-A/MART-1 Antibody (CL12863) - Azide and BSA Free Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody [NBP3-44177]](https://resources.bio-techne.com/images/products/nbp3-44177_mouse-melan-a-mart-1-mab-cl12863-azide-and-bsa-free-282202512324869.jpg "Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody [NBP3-44177]")
Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody [NBP3-44177]
Staining of human kidney shows no positivity as expected.![Melan-A/MART-1 Antibody (CL12863) - Azide and BSA Free Western Blot: Melan-A/MART-1 Antibody [NBP3-44177]](https://resources.bio-techne.com/images/products/nbp3-44177_mouse-melan-a-mart-1-mab-cl12863-azide-and-bsa-free-282202513332395.jpg "Western Blot: Melan-A/MART-1 Antibody [NBP3-44177]")
![Melan-A/MART-1 Antibody (CL12863) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: Melan-A/MART-1 Antibody [NBP3-44177]](https://resources.bio-techne.com/images/products/nbp3-44177_mouse-melan-a-mart-1-mab-cl12863-azide-and-bsa-free-28220251335850.jpg "Immunocytochemistry/ Immunofluorescence: Melan-A/MART-1 Antibody [NBP3-44177]")
![Melan-A/MART-1 Antibody (CL12863) - Azide and BSA Free Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody [NBP3-44177]](https://resources.bio-techne.com/images/products/nbp3-44177_mouse-melan-a-mart-1-mab-cl12863-azide-and-bsa-free-28220251339115.jpg "Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody [NBP3-44177]")
Applications for Melan-A/MART-1 Antibody (CL12863) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
1 ug/ml
Application Notes
For use with IHC-Paraffin, HIER pH 6 retrieval is recommended. For use with Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.
Formulation, Preparation, and Storage
Purification
Protein A purified
Reconstitution
Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.
Formulation
Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Melan-A/MART-1
Alternate Names
Antigen LB39-AA, Antigen SK29-AA, MART-1, MelanA, MLANA
Gene Symbol
MLANA
Additional Melan-A/MART-1 Products
Product Documents for Melan-A/MART-1 Antibody (CL12863) - Azide and BSA Free
Product Specific Notices for Melan-A/MART-1 Antibody (CL12863) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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