Melan-A/MART-1 Antibody (M2-7C10 + M2-9E3) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34680
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-7C10 + M2-9E3) - Azide and BSA Free [NBP2-34680]](https://resources.bio-techne.com/images/products/Melan-A-MART-1-Antibody-M2-7C10-+-M2-9E3-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34680-img0003.jpg "Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-7C10 + M2-9E3) - Azide and BSA Free [NBP2-34680]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
CyTOF-ready, Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Lambda/IgG2b Kappa Clone # M2-7C10 + M2-9E3
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Recombinant full-length hMelan-A/MART-1 protein (M2-7C10; M2-9E3) (Uniprot: Q16655)
Reactivity Notes
Use in Mouse reported in scientific literature (PMID:33857287)
Localization
Cytoplasmic
Specificity
This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This monoclonal antibody labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.
Marker
Melanoma Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Lambda/IgG2b Kappa
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-34245).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Melan-A/MART-1 Antibody (M2-7C10 + M2-9E3) - Azide and BSA Free
Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-7C10 + M2-9E3) - Azide and BSA Free [NBP2-34680]
Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-7C10 + M2-9E3) - Azide and BSA Free [NBP2-34680] - Formalin-fixed, paraffin-embedded human melanoma stained withMelan-A MAb (M2-7C10+M2-9E3). Note cytoplasmic staining of cells.Applications for Melan-A/MART-1 Antibody (M2-7C10 + M2-9E3) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5-1ug/million cells
Immunocytochemistry/ Immunofluorescence
0.5-1ug/ml
Immunohistochemistry-Paraffin
0.5-1ug/ml
Western Blot
0.5-1.0ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml /ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Melan-A/MART-1
Alternate Names
Antigen LB39-AA, Antigen SK29-AA, MART-1, MelanA, MLANA
Gene Symbol
MLANA
Additional Melan-A/MART-1 Products
Product Documents for Melan-A/MART-1 Antibody (M2-7C10 + M2-9E3) - Azide and BSA Free
Product Specific Notices for Melan-A/MART-1 Antibody (M2-7C10 + M2-9E3) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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