Melan-A/MART-1 Antibody (M2-7C10) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33148

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Equine, Rat (Negative)

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Lambda Clone # M2-7C10

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all Melan-A/MART-1 Antibodies »

Product Specifications

Immunogen

Recombinant Melan-A/MART-1 protein (Uniprot: Q16655)

Reactivity Notes

Does not react with Rat.

Localization

Cytoplasmic

Specificity

This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This monoclonal antibody labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.

Marker

Melanoma Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Lambda

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-15197).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Melan-A/MART-1 Antibody (M2-7C10) - Azide and BSA Free

Immunohistochemistry-Frozen: Melan-A/MART-1 Antibody (M2-7C10) - Azide and BSA Free [NBP2-33148]

Immunohistochemistry-Frozen: Melan-A/MART-1 Antibody (M2-7C10) - Azide and BSA Free [NBP2-33148]

Immunohistochemistry-Frozen: Melan-A/MART-1 Antibody (M2-7C10) - Azide and BSA Free [NBP2-33148] - Analysis using the Alexa-Fluor(R)-488 conjugate for this antibody. Melan-A (green) was detected in human skin (melanoma) using Melan-A-AlexaFluor488 antibody (1:40; 1 hour) in PBS. Nuclei were stained with DAPI (blue). Tissue was fixed in acetone. Image from a verified customer review.
Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-7C10) - Azide and BSA Free [NBP2-33148]

Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-7C10) - Azide and BSA Free [NBP2-33148]

Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-7C10) - Azide and BSA Free [NBP2-33148] - Formalin-paraffin human melanoma stained with MART-1 Ab (M2-7C10). Note cytoplasmic staining of cells.
Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-7C10) - Azide and BSA Free [NBP2-33148]

Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-7C10) - Azide and BSA Free [NBP2-33148]

Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-7C10) - Azide and BSA Free [NBP2-33148] - Formalin-fixed, paraffin-embedded human Melanoma stained with Melan-A/MART-1 Antibody (M2-7C10).
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Applications for Melan-A/MART-1 Antibody (M2-7C10) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Frozen

0.5-1ug/ml

Immunohistochemistry-Paraffin

0.5-1ug/ml

Western Blot

0.5-1.0ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Melan-A/MART-1

MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum.

Alternate Names

Antigen LB39-AA, Antigen SK29-AA, MART-1, MelanA, MLANA

Entrez Gene IDs

2315 (Human); 77836 (Mouse); 293890 (Rat)

Gene Symbol

MLANA

Additional Melan-A/MART-1 Products

Product Documents for Melan-A/MART-1 Antibody (M2-7C10) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Melan-A/MART-1 Antibody (M2-7C10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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